Production of d-xylonic acid using a non-recombinant Corynebacterium glutamicum strain.

08:00 EDT 26th July 2018 | BioPortfolio

Summary of "Production of d-xylonic acid using a non-recombinant Corynebacterium glutamicum strain."

It was found that Corynebacterium glutamicum ΔiolR devoid of the transcriptional regulator IolR accumulates high amounts of d-xylonate when cultivated in the presence of d-xylose. Detailed analyses of constructed deletion mutants revealed that the putative myo-inositol 2-dehydrogenase IolG also acts as d-xylose dehydrogenase and is mainly responsible for d-xylonate oxidation in this organism. Process development for d-xylonate production was initiated by cultivating C. glutamicum ΔiolR on defined d-xylose/d-glucose mixtures under batch and fed-batch conditions. The resulting yield matched the theoretical maximum of 1 mol mol and high volumetric productivities of up to 4 g L h could be achieved. Subsequently, a novel one-pot sequential hydrolysis and fermentation process based on optimized medium containing hydrolyzed sugarcane bagasse was developed. Cost-efficiency and abundance of second-generation substrates, good performance indicators, and enhanced market access using a non-recombinant strain open the perspective for a commercially viable bioprocess for d-xylonate production in the near future.


Journal Details

This article was published in the following journal.

Name: Bioresource technology
ISSN: 1873-2976
Pages: 332-339


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A species of CORYNEBACTERIUM isolated from abscesses of warm-blooded animals.

Infections with bacteria of the genus CORYNEBACTERIUM.

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