Advertisement

Topics

Production of d-xylonic acid using a non-recombinant Corynebacterium glutamicum strain.

08:00 EDT 26th July 2018 | BioPortfolio

Summary of "Production of d-xylonic acid using a non-recombinant Corynebacterium glutamicum strain."

It was found that Corynebacterium glutamicum ΔiolR devoid of the transcriptional regulator IolR accumulates high amounts of d-xylonate when cultivated in the presence of d-xylose. Detailed analyses of constructed deletion mutants revealed that the putative myo-inositol 2-dehydrogenase IolG also acts as d-xylose dehydrogenase and is mainly responsible for d-xylonate oxidation in this organism. Process development for d-xylonate production was initiated by cultivating C. glutamicum ΔiolR on defined d-xylose/d-glucose mixtures under batch and fed-batch conditions. The resulting yield matched the theoretical maximum of 1 mol mol and high volumetric productivities of up to 4 g L h could be achieved. Subsequently, a novel one-pot sequential hydrolysis and fermentation process based on optimized medium containing hydrolyzed sugarcane bagasse was developed. Cost-efficiency and abundance of second-generation substrates, good performance indicators, and enhanced market access using a non-recombinant strain open the perspective for a commercially viable bioprocess for d-xylonate production in the near future.

Affiliation

Journal Details

This article was published in the following journal.

Name: Bioresource technology
ISSN: 1873-2976
Pages: 332-339

Links

DeepDyve research library

PubMed Articles [13855 Associated PubMed Articles listed on BioPortfolio]

Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical.

Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemicals including nylon and plasticize...

Enhanced production of d-lactate from mixed sugars in Corynebacterium glutamicum by overexpression of glycolytic genes encoding phosphofructokinase and triosephosphate isomerase.

The use of mixed sugars containing glucose and xylose in lignocellulosic biomass is desirable for the microbial production of chemicals and fuels. We investigated the effect of individual or simultane...

CRISPR interference-mediated metabolic engineering of Corynebacterium glutamicum for homo-butyrate production.

Combinatorial metabolic engineering enabled the development of efficient microbial cell factories for modulating gene expression to produce desired products. Here, we report the combinatorial metaboli...

Reconstruction of TCA cycle in Corynebacterium glutamicum with a genome-scale metabolic network model for trans-4-hydroxyproline production.

trans-4-Hydroxy-l-proline (Hyp) is an abundant component of mammalian collagen, and functions as a chiral synthon for the syntheses of anti-inflammatory drugs in the pharmaceutical industry. Proline 4...

Synthetic Escherichia coli-Corynebacterium glutamicum consortia for l-lysine production from starch and sucrose.

In the biorefinery concept renewable feedstocks are converted to a multitude of value-added compounds irrespective of seasonal or other variations of the complex biomass substrates. Conceptionally, th...

Clinical Trials [3954 Associated Clinical Trials listed on BioPortfolio]

Corynebacterium Spp Bone and Joint Infection: Retrospective Study of Microbiological, Diagnostic and Therapeutic Features

Corynebacterium spp have been considered as innocuous commensals of human skin, but are now accepted as important opportunistic pathogens responsible for various nosocomial infections, esp...

Effects of a Zinc Oxide Underarm Formula on Normal and Injured Skin in Healthy Volunteers

Bothersome odor from the axilla is in most cases caused by Corynebacterium spp. Anti-microbial effects of zinc oxide (ZnO) are well documented. The overall objective of this study is to v...

Fermented Milk Drink on Human Intestinal Microflora

Fermented milk is a popular drink. Recent studies revealed that some fermented milk containing some strains of lactic acid bacteria have health-promoting effects through improvement of the...

The Effect of Dietary Lauric Acid on the Production of the LRH-1 Ligand, Dilauroylphosphatidylcholine

A phosphatidylcholine species enriched with lauric acid at both the sn-1 and sn-2 positions, dilauroylphosphatidylcholine (DLPC), was recently identified as a ligand for the nuclear recept...

Human Challenge Model Refinement With Enterotoxigenic Escherichia Coli Strain B7A

The purpose of this study is to determine the safe and optimal dose and regimen (fasting duration) for administering the challenge ETEC strain B7A, a CS6 expressing ETEC strain. Additiona...

Medical and Biotech [MESH] Definitions

A species in the genus CORYNEBACTERIUM, family Corynebacteriaceae, which is used for industrial production of the amino acid LYSINE. It is closely related to Corynebacterium glutamicum.

A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.

The large scale production of pharmaceutically important and commercially valuable RECOMBINANT PROTEINS.

A species of CORYNEBACTERIUM isolated from abscesses of warm-blooded animals.

Infections with bacteria of the genus CORYNEBACTERIUM.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topic

Recombinant DNA
Recombinant DNA is the formation of a novel DNA sequence by the formation of two DNA strands. These are taken from two different organisms. These recombinant DNA molecules can be made with recombinant DNA technology. The procedure is to cut the DNA of ...


Searches Linking to this Article