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Massively parallel and multiplex blood group genotyping using next-generation-sequencing.

08:00 EDT 6th August 2018 | BioPortfolio

Summary of "Massively parallel and multiplex blood group genotyping using next-generation-sequencing."

Thirty-six blood group systems are listed by the International Society of Blood Transfusion, containing almost 350 antigens. Most of these result from a single nucleotide polymorphism (SNP). Serology is the standard method for blood group typing. However, this technique has some limitations and cannot respond to the growing demand of blood product typing for a large number of antigens. Here we describe a blood group genotyping assay directly from whole blood samples using Next-Generation Sequencing (NGS), allowing the simultaneous identification of 15 SNPs associated with the blood group systems of 95 patients in a single run.

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Name: Clinical biochemistry
ISSN: 1873-2933
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Medical and Biotech [MESH] Definitions

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