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Carotenoids, including β-carotene, are commercially valuable compounds, and their production by engineered Saccharomyces cerevisiae is a promising strategy for their industrial production. Here, to improve β-carotene productivity in engineered S. cerevisiae, a cocktail δ-integration strategy, which involves simultaneous integration of various multi-copy genes, followed by selection of desirable transformants, was applied for modulation of β-carotene production-related genes expression. The engineered strain, YPH499/Mo3Crt79, was constructed by three repeated rounds of cocktail δ-integration using CrtE, CrtYB, and CrtI derived from the yeast, Xanthophyllomyces dendrorhous. The recombinant strain produced 7.3 mg/L of carotenoids in 48 h and 52.3 mg/L of β-carotene in 96 h, which were greater values than those achieved by CrtE, CrtYB, and CrtI co-overexpressing strains. Therefore, repeated cocktail δ-integration was effective in improving carotenoid productivity in S. cerevisiae and could be a promising technique for constructing metabolically engineered S. cerevisiae capable of producing bio-based chemicals.
This article was published in the following journal.
Name: Bioresource technology
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The integration of exogenous DNA into the genome of an organism at sites where its expression can be suitably controlled. This integration occurs as a result of homologous recombination.
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The expression of a gene in an abnormal place, or at an abnormal time in an organism. Ectopic Gene Expression is often induced artificially by genetic techniques.
A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.
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