Track topics on Twitter Track topics that are important to you
Disseminated cancer cells in malignant ascites possess unique properties that differ from primary tumors. However, the biological features of ascites tumor cells (ATCs) have not been fully investigated. By analyzing ascites fluid from 65 gastrointestinal cancer patients, the distinguishing characteristics of ATCs were identified. High frequency of CD44+ cells was observed in ATCs using flow cytometry (n=48). Multiplex quantitative PCR (n=15) showed higher gene expression of epithelial-mesenchymal transition (EMT)-related genes and transforming growth factor beta (TGF-beta)-related genes in ATCs than in the primary tissues. Immunohistochemistry (n=10) showed that ATCs also had much higher expression of phosphorylated SMAD2 than that in the corresponding primary tissues. TGF-beta1 was detected in all cases of malignant ascites by enzyme-linked immunoassay (n=38), suggesting the possible interaction of ATCs and the ascites microenvironment. In vitro experiments revealed that these ATC properties were maintained by TGF- beta1 in cultured ATCs (n=3). Here, we showed that ATCs revealed high frequencies of CD44 and possessed distinct EMT features from primary tissues, that were mainly maintained by TGF-beta1 in the ascites. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Cancer science
Genetic program regulating epithelial-mesenchymal transition is activated at the early stages of embryogenesis. Abnormal karyotype of the embryo disrupts the fundamental process of epithelial-mesenchy...
The histogenesis process of pleomorphic adenoma (PA) of salivary gland continues to remain a controversial subject. In this neoplasm, transition occurs from an epithelial or myoepithelial cell to a st...
Cell-free DNA (cfDNA) has been a research hotspot in molecular tumor profiling. In advanced gastric cancer patients, malignant pleural effusion (MPE) and ascites provide a wealth of tumor cells that c...
Gastric cancer is a common malignancy worldwide. The prognosis of early stage gastric cancer patients has significantly improved in recent years. However, in progressive stage gastric cancer patients,...
The most life-threatening step during malignant tumor progression is reached when cancer cells leave the primary tumor mass and seed metastasis in distant organs. To infiltrate the surrounding tissue ...
This is a randomized phase IIIb study investigating the treatment of malignant ascites due to epithelial cancer (carcinomas) with the trifunctional antibody catumaxomab. In order to make t...
Malignant ascites is a severe complication of many types of human cancer. Animal and clinical analyses have shown that angiogenesis plays a critical role in the formation of malignant asci...
The purpose of this study is to determine the effectiveness of using Bevacizumab in the prevention of recurrent malignant ascites. Ascites is a debilitating and unpleasant complication of...
This phase II single arm, open-label study investigate the safety of a second cycle of catumaxomab in patients with malignant ascites due to carcinoma, requiring their first therapeutic pu...
Malignant pleural effusion and/ or malignant ascites is generally defined by presence of malignant cells in the effusion fluid. The first-line therapies are mostly intrusive, medically dem...
Phenotypic changes of EPITHELIAL CELLS to MESENCHYME type, which increase cell mobility critical in many developmental processes such as NEURAL TUBE development. NEOPLASM METASTASIS and DISEASE PROGRESSION may also induce this transition.
A malignant neoplasm that contains elements of carcinoma and sarcoma so extensively intermixed as to indicate neoplasia of epithelial and mesenchymal tissue. (Stedman, 25th ed)
A malignant neoplasm occurring in young children, primarily in the liver, composed of tissue resembling embryonal or fetal hepatic epithelium, or mixed epithelial and mesenchymal tissues. (Stedman, 25th ed)
A highly-conserved family of basic helix-loop-helix (bHLH) transcription factors. They function as dimers with other bHLH proteins and bind E-BOX ELEMENTS to control gene expression during EMBRYOGENESIS and the EPITHELIAL-MESENCHYMAL TRANSITION.
A transcription factor family characterized by the presence of several C-terminal CYS2-HIS2 ZINC FINGERS. They function in many developmental processes including the induction of the EPITHELIAL-MESENCHYMAL TRANSITION; maintenance of embryonic MESODERM; growth arrest, CELL SURVIVAL; and CELL MIGRATION.
Cancer is not just one disease but many diseases. There are more than 100 different types of cancer. Most cancers are named for the organ or type of cell in which they start - for example, cancer that begins in the colon is called colon cancer; cancer th...
Bladder Cancer Brain Cancer Breast Cancer Cancer Cervical Cancer Colorectal Head & Neck Cancers Hodgkin Lymphoma Leukemia Lung Cancer Melanoma Myeloma Ovarian Cancer Pancreatic Cancer ...
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...