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Epithelial-mesenchymal transition is activated in CD44 positive malignant ascites tumor cells of gastrointestinal cancer.

08:00 EDT 24th August 2018 | BioPortfolio

Summary of "Epithelial-mesenchymal transition is activated in CD44 positive malignant ascites tumor cells of gastrointestinal cancer."

Disseminated cancer cells in malignant ascites possess unique properties that differ from primary tumors. However, the biological features of ascites tumor cells (ATCs) have not been fully investigated. By analyzing ascites fluid from 65 gastrointestinal cancer patients, the distinguishing characteristics of ATCs were identified. High frequency of CD44+ cells was observed in ATCs using flow cytometry (n=48). Multiplex quantitative PCR (n=15) showed higher gene expression of epithelial-mesenchymal transition (EMT)-related genes and transforming growth factor beta (TGF-beta)-related genes in ATCs than in the primary tissues. Immunohistochemistry (n=10) showed that ATCs also had much higher expression of phosphorylated SMAD2 than that in the corresponding primary tissues. TGF-beta1 was detected in all cases of malignant ascites by enzyme-linked immunoassay (n=38), suggesting the possible interaction of ATCs and the ascites microenvironment. In vitro experiments revealed that these ATC properties were maintained by TGF- beta1 in cultured ATCs (n=3). Here, we showed that ATCs revealed high frequencies of CD44 and possessed distinct EMT features from primary tissues, that were mainly maintained by TGF-beta1 in the ascites. This article is protected by copyright. All rights reserved.

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Name: Cancer science
ISSN: 1349-7006
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