Analytical methods for the study of bioactive compounds from medicinally used Echinacea species.

08:00 EDT 6th August 2018 | BioPortfolio

Summary of "Analytical methods for the study of bioactive compounds from medicinally used Echinacea species."

Echinacea purpurea (L.) Moench, Echinacea angustifolia DC. var. angustifolia and Echinacea pallida (Nutt.) Nutt. are frequently used as medicinal plants and their preparations are among the most widely used herbal medicines. The extracts from these species have shown a highly complex chemical composition, including polar compounds (caffeic acid derivatives, CADs), non-polar compounds (alkylamides and acetylenic secondary metabolites; essential oil) and high molecular weight constituents (polysaccharides and glycoproteins). All these chemical classes of compounds have demonstrated to possess interesting biological activities. In the light of all the above, this paper is focused on the analytical techniques, including sample preparation tools and chromatographic procedures, for the chemical analysis of bioactive compounds in medicinally used Echinacea species. Since sample preparation is considered to be a crucial step in the development of analytical methods for the determination of constituents present in herbal preparations, the strength and weakness of different extraction techniques are discussed. As regards the analysis of compounds present in Echinacea plant material and derivatives, the application of different techniques, mainly HPLC, HPLC-ESI-MS, HPLC-ESI-MS/MS, HPCE, HPTLC and GC, is discussed in detail. The strength, weakness and applicability of the different separation tools are stated.


Journal Details

This article was published in the following journal.

Name: Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Pages: 443-477


DeepDyve research library

PubMed Articles [20935 Associated PubMed Articles listed on BioPortfolio]

High-Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry.

Bioactive lipids represent critical intra- and intercellular signaling molecules, and have been implicated in both physiologic homeostasis and disease pathology. Measurement of bioactive lipids is vit...

Antiinflammation constituents from Curcuma zedoaroides.

Curcuma zedoaroides, a Zingiberaceae species, has been used for snake bite antidote and wound care in Thailand. Seven compounds were isolated from the bioactive chloroform extract consisted of one new...

Request Pattern, Pre-Analytical and Analytical Conditions of Urinalysis in Primary Care: Lessons from a One-Year Large-Scale Multicenter Study.

To study the urinalysis request, pre-analytical sample conditions, and analytical procedures.

Synthesis, X-ray diffraction study and pharmacological evaluation of 3-amino-4-methylthiophene-2-acylcarbohydrazones.

N-acylhydrazone is an interesting privileged structure that has been used in the molecular design of a myriad of bioactive compounds. In order to identify new antinociceptive drug candidates, we descr...

Instrumental analysis of microplastics-benefits and challenges.

There is a high demand for easy, cheap, comparable, and robust methods for microplastic (MP) analysis, due to the ever-increasing public and scientific interest in (micro-) plastic pollution in the en...

Clinical Trials [1860 Associated Clinical Trials listed on BioPortfolio]

Content, Bioavailability and Health Effects of Trace Elements and Bioactive Components in Organic Agricultural Systems

Trace elements, bioactive secondary metabolites and vitamins are among the most important quality parameters in plants. Yet, very little information is available on their content, bioavail...

Lipids and Other Bioactive Compounds of Human Breast Milk

The biochemical differences in the composition of breast milk and formula have not been profoundly established in all compound groups. Increased understanding of breast milk composition, t...

Bioactive Glass and Platelet Rich Fibrin in Intrabony Defects

Background: Platelet-rich fibrin (PRF) and bioactive glass putty has been shown to be effective in promoting reduction in probing depth, gain in clinical attachment, and defect fill in int...

Effect of Supplementation of Bioactive Compounds on the Energy Metabolism of People Living With HIV / AIDS

Among the many changes associated with the impact of HIV and the long-term use of antiretroviral therapy, metabolics are important because they are important risk factors for the developme...

Post-Market Clinical Investigation of NanoFUSE® Bioactive Matrix

This purpose of this study is to determine fusion rates and compare clinical outcomes of the NanoFUSE® Bioactive Matrix during post-market clinical use.

Medical and Biotech [MESH] Definitions

Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.

Research techniques that focus on study designs and data gathering methods in human and animal populations.

Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.

Methods of preparing cells or tissues for examination and study of their origin, structure, function, or pathology. The methods include preservation, fixation, sectioning, staining, replica, or other technique to allow for viewing using a microscope.

Compounds formed by condensation of secologanin with tryptamine resulting in a tetrahydro-beta-carboline which is processed further to a number of bioactive compounds. These are especially found in plants of the APOCYNACEAE; LOGANIACEAE; and RUBIACEAE families.

Quick Search


DeepDyve research library

Relevant Topic

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...

Searches Linking to this Article