Histamine H receptor mediates chemotaxis of human lung mast cells.

08:00 EDT 24th August 2018 | BioPortfolio

Summary of "Histamine H receptor mediates chemotaxis of human lung mast cells."

The diverse effects of histamine are mediated by discrete histamine receptors. The principal repository of histamine in the body is the mast cell. However, the effects of histamine on mast cells, especially those of human origin, have not been fully elucidated. In this study, the expression of histamine receptors in human lung mast cells was evaluated. Moreover, the effects of histamine receptor engagement on both mediator release and chemotaxis were investigated. Mast cells were isolated and purified from human lung tissue. Histamine receptor expression was determined by RT-PCR and q-PCR. Both methods for the detection of histamine receptors were in accordance and human lung mast cells expressed mRNA for histamine H and histamine H receptors, variably expressed histamine H receptor but did not express histamine H receptor. The effects of selective histamine receptor agonists on the release of both pre-formed (histamine) and newly-synthesized (cysteinyl-leukotriene, prostaglandin D) mediators were investigated. None of the agonists tested had any direct effects on mediator release. None of the agonists modulated release stimulated by anti-IgE. Further studies showed that histamine induced migration of mast cells. Chemotaxis appeared to be mediated by the histamine H receptor since JNJ28610244 (H agonist) was chemotactic for mast cells whereas 2-(2-pyridyl) ethylamine (H agonist) was not. Furthermore, the selective histamine H receptor antagonist, JNJ7777120, effectively reversed the chemotaxis of mast cells induced by JNJ28610244. Overall, these experiments identify the histamine H receptor as chemotactic for human lung mast cells. This mechanism might influence mast cell accumulation in the lung.


Journal Details

This article was published in the following journal.

Name: European journal of pharmacology
ISSN: 1879-0712


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