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Curvature as an important topological parameter of 3D extra-cellular matrix has drawn growing attention in recent years. But the underlying mechanism that curvature influences cell behaviors has remained unknown. In this study, we seeded cells on semi-cylindrical and hemispheric surfaces and tested cell alignment and polarization. We found that the surface curvature has profound effect on cell behaviors. With the decrease of diameter of the cylinder/sphere (i.e. increase of curvature), the cells would more preferentially align and polarize with large aspect ratio in the axial/peripheral direction. And the behaviors of the alignment and polarization were position-dependent. For example, at the end of the cylinder, the cells preferred to align circumferentially; while in the interior region, the cells preferred to align in the axial direction. We showed that the cell polarization and alignment were closely correlated with the in-plane stresses in cell layer. That is, the cell polarization and alignment were controlled by the maximum shear stress, which drove cells to align and polarize along the maximum principal stress. The curvature could influence the magnitude of the maximum shear stress and thus regulate cell behaviors. This study provided important insights into the mechanisms of surface curvature influencing cell behaviors in tissue morphogenesis. In addition, our theory of the stress dependent cellular polarity provides a generalized interpretation of the curvature and edge effects which might be extended to understand other steric effects in cell behaviors.
This article was published in the following journal.
Name: Journal of the mechanical behavior of biomedical materials
Mesenchymal cell migration relies on the coordinated regulation of the actin and microtubule networks that participate in polarized cell protrusion, adhesion, and contraction. During collective migrat...
During wound healing, cells migrate with electrotactic bias as a collective entity. Unlike the case of EF-induced single cell migration, the sensitivity of electrotactic response depends primarily on ...
Both dorsal and ventral visual pathways harbor several areas sensitive to gradients of binocular disparity (i.e. higher-order disparity). While a wealth of information exists about disparity processin...
Cell migration, critical to numerous biological processes, can be guided by surface topography. Studying the effects of topography on cell migration is valuable for enhancing our understanding of dire...
Collective invasion drives the spread of multicellular cancer groups, into the normal tissue surrounding several epithelial tumors. Collective invasion recapitulates various aspects of the multicellul...
A study to assess the utility of human polarization pattern perception for the detection, diagnosis and monitoring of eye disease
The goal of this study is to evaluate patient comfort during compression with a standard flat mammographic paddle and an investigational curved paddle. This is a study that will be perform...
The aim of this study is to evaluate postoperative knee function after total knee arthroplasty performed according to the anatomical alignment and compare these results to those of a match...
Background: - Direct current (DC) brain polarization is a technique in which very weak electricity is applied to the head. Doctors have used DC polarization for many years on pati...
The purpose of this study is to see the correlation of polarization and the expresison of chemokine receptors of T help cells after allogeneic hematopoietic stem cell transplantation and c...
A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
Dyneins that are responsible for intracellular transport, MITOSIS, cell polarization, and movement within the cell.
Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.
Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labeled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations.
Either of a pair of compound bones forming the lateral (left and right) surfaces and base of the skull which contains the organs of hearing. It is a large bone formed by the fusion of parts: the squamous (the flattened anterior-superior part), the tympanic (the curved anterior-inferior part), the mastoid (the irregular posterior portion), and the petrous (the part at the base of the skull).
Stress is caused by your perception of situations around you and then the reaction of your body to them. The automatic stress response to unexpected events is known as 'fight or flight'. Discovered by Walter Cannon in 1932, it is the release of h...
Adhd Anorexia Depression Dyslexia Mental Health Psychiatry Schizophrenia Stress Mental health, although not being as obvious as physical health, is very important, causing great unhappiness to those affected, causing add...