Collective cell polarization and alignment on curved surfaces.
Summary of "Collective cell polarization and alignment on curved surfaces."
Curvature as an important topological parameter of 3D extra-cellular matrix has drawn growing attention in recent years. But the underlying mechanism that curvature influences cell behaviors has remained unknown. In this study, we seeded cells on semi-cylindrical and hemispheric surfaces and tested cell alignment and polarization. We found that the surface curvature has profound effect on cell behaviors. With the decrease of diameter of the cylinder/sphere (i.e. increase of curvature), the cells would more preferentially align and polarize with large aspect ratio in the axial/peripheral direction. And the behaviors of the alignment and polarization were position-dependent. For example, at the end of the cylinder, the cells preferred to align circumferentially; while in the interior region, the cells preferred to align in the axial direction. We showed that the cell polarization and alignment were closely correlated with the in-plane stresses in cell layer. That is, the cell polarization and alignment were controlled by the maximum shear stress, which drove cells to align and polarize along the maximum principal stress. The curvature could influence the magnitude of the maximum shear stress and thus regulate cell behaviors. This study provided important insights into the mechanisms of surface curvature influencing cell behaviors in tissue morphogenesis. In addition, our theory of the stress dependent cellular polarity provides a generalized interpretation of the curvature and edge effects which might be extended to understand other steric effects in cell behaviors.
Affiliation
Journal Details
This article was published in the following journal.
Name: Journal of the mechanical behavior of biomedical materials
ISSN: 1878-0180
Pages: 330-339
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/30196189
- DOI: http://dx.doi.org/10.1016/j.jmbbm.2018.08.014
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Medical and Biotech [MESH] Definitions
Freeze Etching
A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
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Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.
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Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labeled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations.
Temporal Bone
Either of a pair of compound bones forming the lateral (left and right) surfaces and base of the skull which contains the organs of hearing. It is a large bone formed by the fusion of parts: the squamous (the flattened anterior-superior part), the tympanic (the curved anterior-inferior part), the mastoid (the irregular posterior portion), and the petrous (the part at the base of the skull).
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