Unraveling the genetic basis of fast L-arabinose consumption on top of recombinant xylose-fermenting Saccharomyces cerevisiae.

08:00 EDT 10th September 2018 | BioPortfolio

Summary of "Unraveling the genetic basis of fast L-arabinose consumption on top of recombinant xylose-fermenting Saccharomyces cerevisiae."

One major challenge in the bioconversion of lignocelluloses into ethanol is to develop Saccharomyces cerevisiae strains that can utilize all available sugars in biomass hydrolysates, especially the D-xylose and L-arabinose that cannot be fermented by the S. cerevisiae strain naturally. Here, we integrated an L-Arabinose Utilization Cassette (AUC) into the genome of an efficient D-xylose fermenting industrial diploid S. cerevisiae strain CIBTS0735 to make strain CIBTS1972. After evolving on arabinose, CIBTS1974 with excellent fermentation capacity was obtained. A comparison between genome sequences of strains CIBTS1974 and CIBTS1972 revealed that the copy number of the AUC had increased from 1 to 12. We then constructed the AUC null mutant CIBTS1975 and gradually rescued the L-arabinose utilization defect by integrating AUC iteratively. On the other hand, the parental strain CIBTS0735 was able to acquire the same performance as CIBTS1974 by the direct introduction of 12 copies of the AUC; the performance was further improved by adding two more copies. Besides, we found that not the two transporters present in the AUC were both needed during L-arabinose utilization, Gal2 was necessary and Stp2 was not essential. We have described for the first time that a high copy number of AUC is sufficient for the strain to metabolize L-arabinose efficiently independent of evolution. This article is protected by copyright. All rights reserved.


Journal Details

This article was published in the following journal.

Name: Biotechnology and bioengineering
ISSN: 1097-0290


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Medical and Biotech [MESH] Definitions

The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional regulatory regions, or a new chimeric gene product. ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. ARTIFICIAL GENE FUSION is carried out in vitro by RECOMBINANT DNA technology.

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