Track topics on Twitter Track topics that are important to you
Collagen plays a decisive role as a functional substrate in tissue engineering. In particular, the rigidity of the collagen influences the behaviour of the attached cells. Thus, modification and controlled adjustment of collagen's characteristics are essential. To this end, controlled exposure to ultraviolet (UV) light is a promising process because it can be temporally and spatially well defined. In this study, we investigated the effect of UV exposure on surface supported single collagen fibrils in situ. This procedure allowed for a direct comparison between the untreated and modified states of type I collagen. Atomic force microscopy was used to map the mechanical properties. Exposure to UV light was used to influence the mechanical properties of the fibrils in varied liquid environments (deionized water and phosphate-buffered saline (PBS)). The results led to the assumption that combined UV/thermal treatment in deionized water continuously lowers the elastic modulus. In contrast, experiments performed in PBS-based solutions in combination with UV-B and UV-C light or thermal treatment up to 45 °C suggested an increase in the modulus within the first 30-40 min that subsequently decreased again. Thus, the wavelength, exposure, temperature, and chemical environment are relevant parameters that need to be controlled when modifying collagen using UV light.
This article was published in the following journal.
Name: Journal of the mechanical behavior of biomedical materials
The goal of mechanobiology is to understand the links between changes in the physical properties of living cells and normal physiology and disease. This requires mechanical measurements that have appr...
We report how individual defects affect single graphene nanoribbons by scanning tunneling and atomic force microscopy pulling experiments simultaneously accessing their electrical and mechanical prope...
Identification of nanoparticles and nanosystems into cells and biological matrices is a hot research topic in nanobiotechnologies. Because of their capability to map physical properties (mechanical, e...
Cell mechanics plays an important role in regulating the physiological activities of cells. The advent of atomic force microscopy (AFM) provides a novel powerful instrument for quantifying the mechani...
Single-molecule force spectroscopy makes it possible to measure the mechanical strength of single noncovalent receptor-ligand-type bonds. A major challenge in this technique is to ensure that measurem...
Inter-individual variability in energetic cost of running may be partly explained by lower limbs mechanical characteristics. The transposition of muscle mechanical model with two component...
The purpose of this study is to measure the mechanical properties of healing Achilles tendons in humans after early controlled weightbearing, compared with a control group in a randomized,...
The goal of this study is the evaluation of an atomic magnetometer that is practical for human biomagnetism studies. We have built a device that has the necessary sensitivity, and will be ...
Ehlers-Danlos Syndrome (EDS) is an inherited disease of collagen, found in connective tissues, such as skin. EDS patients suffer from joint and skin problems (skin hyperextensibility, join...
The buccal and lingual surface of the 23 participants' crowns will be randomly assigned to receive scaling and root planing with either Teflon scalers, or non-Teflon scalers after implant ...
Scanning microscopy in which a very sharp probe is employed in close proximity to a surface, exploiting a particular surface-related property. When this property is local topography, the method is atomic force microscopy (MICROSCOPY, ATOMIC FORCE), and when it is local conductivity, the method is scanning tunneling microscopy (MICROSCOPY, SCANNING TUNNELING).
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
The properties and processes of materials that affect their behavior under force.
Nanometer-sized tubes composed mainly of CARBON. Such nanotubes are used as probes for high-resolution structural and chemical imaging of biomolecules with ATOMIC FORCE MICROSCOPY.
Non-invasive imaging methods based on the mechanical response of an object to a vibrational or impulsive force. It is used for determining the viscoelastic properties of tissue, and thereby differentiating soft from hard inclusions in tissue such as microcalcifications, and some cancer lesions. Most techniques use ultrasound to create the images - eliciting the response with an ultrasonic radiation force and/or recording displacements of the tissue by Doppler ultrasonography.