Automated Bright Field Segmentation of Cells and Vacuoles Using Image Processing Technique.

08:00 EDT 19th September 2018 | BioPortfolio

Summary of "Automated Bright Field Segmentation of Cells and Vacuoles Using Image Processing Technique."

Understanding the mechanisms and other variants of programmed cell death will help provide deeper insight into various disease processes. Although complex procedures are required to distinguish each type of cell death, the formation of vacuoles is one of the important features in some process of cell death under different conditions. Thus, monitoring and counting the number of vacuoles and the ratio of cells with vacuoles is a commonly used method to indicate and quantify the efficacy of the therapy. Several studies have shown that image processing can provide a quick, convenient and precise mean of performing cell detection. Hence, this study uses an image processing technique to detect and quantify vacuolated cells without the need for dyes. The system both counts the number of vacuolated cells and determines the ratio of cells with vacuoles. The performance of the proposed image processing system was evaluated using 38 images. It has been shown that a strong correlation exists between the automated counts and the manual counts. Furthermore, the absolute percentage errors between automated counts and manual counts for cell detection and vacuolated cell detection using data pooled from all images are 3.61 and 3.33%, respectively. A user-friendly graphical user interface (GUI) is also developed and freely available for download, providing researchers in biomedicine with a more convenient instrument for vacuolization analysis.


Journal Details

This article was published in the following journal.

Name: Cytometry. Part A : the journal of the International Society for Analytical Cytology
ISSN: 1552-4930


DeepDyve research library

PubMed Articles [22973 Associated PubMed Articles listed on BioPortfolio]

Simplified, automated methods for assessing pixel intensities of fluorescently-tagged drugs in cells.

Assessing the cytoplasmic uptake of fluorescently-tagged drugs in heterogeneous cell types currently involves time-consuming manual segmentation of confocal microscopy images. We developed a set of me...

Theoretical framework of statistical noise in scanning transmission electron microscopy.

Statistical noise, or shot noise, dominates experimental image quality in scanning transmission electron microscopy because efficiencies of recent detectors are close to ideal. We establish a general ...

A Novel Extension to Fuzzy Connectivity for Body Composition Analysis: Applications in Thigh, Brain, and Whole Body Tissue Segmentation.

Magnetic resonance imaging (MRI) is the non-invasive modality of choice for body tissue composition analysis due to its excellent soft tissue contrast and lack of ionizing radiation. However, quantifi...

Image segmentation by graph cut for radiation images of small animal blood vessels.

Synchrotron radiation (SR) based X-ray imaging is an attractive method for analyzing biomedical structure. However, despite its many advantages, there are few gold standards in image processing method...

Image Segmentation for Intensity Inhomogeneity in Presence of High Noise.

Automated segmentation of fine objects details in a given image is becoming of crucial interest in different imaging fields. In this paper, we propose a new variational level-set model for both global...

Clinical Trials [9139 Associated Clinical Trials listed on BioPortfolio]

Prospective Study for Transplant Optimization Using Functional Imaging (TROFI)

The objective of this study is to detect BOS in an early stage by using the outcome parameters generated by Functional Respiratory Imaging (FRI). Robust and automated segmentation algorith...

OCT Image Quality Performance Evaluation

To evaluate the image qualities and segmentation performance of Topcon's DRI OCT Triton devices using two light sources.

Is There a Pancreatic Segmentation Based on the Pancreatic Duct Branching?

Limited pancreatic resections are increasingly performed, but the rate of postoperative fistula is higher than after classical resections. Pancreatic segmentation, anatomically and radiolo...

Feasibility Pilot of Bright Light in the Intensive Care Unit

To evaluate the feasibility of providing daytime bright light in the ICU in a pilot randomized controlled trial.

Exercise and Bright Light Treatment: Effects on Body Weight and Composition

Bright light therapy has been used to safely and effectively treat conditions such as Seasonal Affective Disorder and to regularize sleep in patients with circadian rhythm disorder. Based ...

Medical and Biotech [MESH] Definitions

A method of delineating blood vessels by subtracting a tissue background image from an image of tissue plus intravascular contrast material that attenuates the X-ray photons. The background image is determined from a digitized image taken a few moments before injection of the contrast material. The resulting angiogram is a high-contrast image of the vessel. This subtraction technique allows extraction of a high-intensity signal from the superimposed background information. The image is thus the result of the differential absorption of X-rays by different tissues.

Relatively bright light, or the dazzling sensation of relatively bright light, which produces unpleasantness or discomfort, or which interferes with optimal VISION, OCULAR. (Cline et al., Dictionary of Visual Science, 4th ed)

Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.

The production of an image obtained by cameras that detect the radioactive emissions of an injected radionuclide as it has distributed differentially throughout tissues in the body. The image obtained from a moving detector is called a scan, while the image obtained from a stationary camera device is called a scintiphotograph.

The engulfing of liquids by cells by a process of invagination and closure of the cell membrane to form fluid-filled vacuoles.

Quick Search


DeepDyve research library

Searches Linking to this Article