Advertisement

Topics

Automated morphological feature assessment for zebrafish embryo developmental toxicity screens.

08:00 EDT 8th October 2018 | BioPortfolio

Summary of "Automated morphological feature assessment for zebrafish embryo developmental toxicity screens."

Detection of developmental phenotypes in zebrafish embryos typically involves a visual assessment and scoring of morphological features by an individual researcher. Subjective scoring could impact results and be of particular concern when phenotypic effect patterns are also used as a diagnostic tool to classify compounds. Here we introduce a quantitative morphometric approach based on image analysis of zebrafish embryos. A software called FishInspector was developed to detect morphological features from images collected using an automated system to position zebrafish embryos. The analysis was verified and compared with visual assessments of three participating laboratories using three known developmental toxicants (methotrexate, dexamethasone and topiramate) and two negative compounds (loratadine and glibenclamide). The quantitative approach exhibited higher sensitivity and made it possible to compare patterns of effects with the potential to establish a grouping and classification of developmental toxicants. Our approach improves the robustness of phenotype scoring and reliability of assay performance and, hence, is anticipated to improve the predictivity of developmental toxicity screening using the zebrafish embryo.

Affiliation

Journal Details

This article was published in the following journal.

Name: Toxicological sciences : an official journal of the Society of Toxicology
ISSN: 1096-0929
Pages:

Links

DeepDyve research library

PubMed Articles [16445 Associated PubMed Articles listed on BioPortfolio]

Toxicity effects of captan on different life stages of zebrafish (Danio rerio).

The objective of this study was to evaluate the toxicity and developmental effects of captan on different life stages (embryo and adult) of zebrafish (Danio rerio). The results showed that the 96-h le...

High throughput automated detection of axial malformations in Medaka embryo.

Fish embryo models are widely used as screening tools to assess the efficacy and/or toxicity of chemicals. This assessment involves the analysis of embryo morphological abnormalities. In this article,...

Developmental toxicity of triclocarban in zebrafish (Danio rerio) embryos.

Triclocarban (TCC), which is used as an antimicrobial agent in personal care products, has been widely detected in aquatic ecosystems. However, the consequence of TCC exposure on embryo development is...

Exposure to DBP induces the toxicity in early development and adverse effects on cardiac development in zebrafish (Danio rerio).

Dibutyl phthalate (DBP) is one of the most ubiquitous plasticizers used worldwide and has been frequently detected in soil, water, atmosphere, and other environmental media. DBP has become a ubiquitou...

Zebrafish embryo and acute fish toxicity test show similar sensitivity for narcotic compounds.

The zebrafish embryo test has been discussed as an alternative test system to provide data on acute fish toxicity required by diverse regulations. A meta-analysis of zebrafish embryo acute toxicity (Z...

Clinical Trials [6048 Associated Clinical Trials listed on BioPortfolio]

Xenotransplantation of Primary Cancer Samples in Zebrafish Embryos

The study consists in a co-clinical trial by using zebrafish embryos. Specifically, an observational prospective clinical trial on patients operated of epato-biliar-pancreatic cancers and ...

TICON-Day 3, Time Lapse Versus Conventional Method in Day 3 Embryo Culture and Assessment

The main purpose of the study is to evaluate the overall clinical value of GERI+ as an integrated embryo culture and assessment system, providing an undisturbed culture environment, contin...

Single Embryo TrAnsfeR of Eupoid Embryo

The purpose of the current study is to evaluate the effect of preimplantation genetic screening (PGS) by next generation sequencing (NGS) compared to standard morphological assessment of e...

Interest of Morphokinetic Parameters of Early Embryonic Development in the Birth Rates in Vitro Fertilization

In France, nearly 3% of children are conceived by the technique of Assisted Reproductive Medicine, of which nearly 70% by in vitro fertilization (IVF). The rates of clinical pregnancy by o...

Functional MRI Study in Children With a Developmental Coordination Disorder (DCD) and/or Developmental Dyslexia (DD)

All the studies underlined the high frequency of co-morbid associations in specific learning disorders. Understanding the reasons for these associations could enable us to determine the ce...

Medical and Biotech [MESH] Definitions

The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.

The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.

Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).

Experimentation on, or using the organs or tissues from, a human or other mammalian conceptus during the prenatal stage of development that is characterized by rapid morphological changes and the differentiation of basic structures. In humans, this includes the period from the time of fertilization to the end of the eighth week after fertilization.

Morphological findings useful in differentiation and classification of results in CYTODIAGNOSIS and related techniques.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topic

Assays
An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity.  This can be a drug or biochemical substance or a cell in an organism or organic sample. ...


Searches Linking to this Article