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The soil-borne bacterium Ralstonia solanacearum invades the roots and colonizes the intercellular species and then the xylem. The expression of lecM, encoding a lectin LecM, is induced by an OmpR family response regulator HrpG in R. solanacearum strain OE1-1. LecM contributes to the attachment of strain OE1-1 to host cells of intercellular spaces. OE1-1 produces methyl 3-hydroxymyristate (3-OH MAME) through a methyltransferase (PhcB) and extracellularly secretes the chemical as a quorum-sensing (QS) signal, which activates QS. The expression of lecM is also induced by PhcA virulence regulator functioning through QS, and the resultant LecM is implicated in the QS-dependent production of major exopolysaccharide EPS I and the aggregation of OE1-1 cells. To investigate LecM functions in QS, we analysed the transcriptome of R. solanacearum strains generated by RNA sequencing technology. In the lecM mutant, the expression of positively QS-regulated genes (by > 90%) and the expression of negatively QS-regulated genes (by ~60%) was downregulated and upregulated, respectively. However, phcB and phcA in the lecM mutant were expressed at levels similar to those in strain OE1-1. The lecM mutant produced significantly less ralfuranone and exhibited a significantly greater swimming motility, which are positively and negatively regulated by QS, respectively. In addition, the extracellular 3-OH MAME content of lecM mutant was significantly lower than that of OE1-1. The application of 3-OH MAME increased more EPS I production in the phcB-deleted mutant and strain OE1-1 than in the lecM mutant. Thus, QS-dependent produced LecM contributes to the QS signalling pathway. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Molecular plant pathology
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A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
A species of Ralstonia previously classed in the genera PSEUDOMONAS and BURKHOLDERIA. It is an important plant pathogen.
Complement activation triggered by the interaction of microbial POLYSACCHARIDES with serum MANNOSE-BINDING LECTIN resulting in the activation of MANNOSE-BINDING PROTEIN-ASSOCIATED SERINE PROTEASES. As in the classical pathway, MASPs cleave COMPLEMENT C4 and COMPLEMENT C2 to form C3 CONVERTASE (C4B2A) and the subsequent C5 CONVERTASE (C4B2A3B) leading to cleavage of COMPLEMENT C5 and assembly of COMPLEMENT MEMBRANE ATTACK COMPLEX.
The type species in the genus RALSTONIA. It is often found in the hospital ward as a contaminant of antiseptic and disinfectant solutions.
A specific mannose-binding member of the collectin family of lectins. It binds to carbohydrate groups on invading pathogens and plays a key role in the MANNOSE-BINDING LECTIN COMPLEMENT PATHWAY.
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