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Contribution of a lectin, LecM, to the quorum sensing signalling pathway of Ralstonia solanacearum strain OE1-1.

08:00 EDT 12th October 2018 | BioPortfolio

Summary of "Contribution of a lectin, LecM, to the quorum sensing signalling pathway of Ralstonia solanacearum strain OE1-1."

The soil-borne bacterium Ralstonia solanacearum invades the roots and colonizes the intercellular species and then the xylem. The expression of lecM, encoding a lectin LecM, is induced by an OmpR family response regulator HrpG in R. solanacearum strain OE1-1. LecM contributes to the attachment of strain OE1-1 to host cells of intercellular spaces. OE1-1 produces methyl 3-hydroxymyristate (3-OH MAME) through a methyltransferase (PhcB) and extracellularly secretes the chemical as a quorum-sensing (QS) signal, which activates QS. The expression of lecM is also induced by PhcA virulence regulator functioning through QS, and the resultant LecM is implicated in the QS-dependent production of major exopolysaccharide EPS I and the aggregation of OE1-1 cells. To investigate LecM functions in QS, we analysed the transcriptome of R. solanacearum strains generated by RNA sequencing technology. In the lecM mutant, the expression of positively QS-regulated genes (by > 90%) and the expression of negatively QS-regulated genes (by ~60%) was downregulated and upregulated, respectively. However, phcB and phcA in the lecM mutant were expressed at levels similar to those in strain OE1-1. The lecM mutant produced significantly less ralfuranone and exhibited a significantly greater swimming motility, which are positively and negatively regulated by QS, respectively. In addition, the extracellular 3-OH MAME content of lecM mutant was significantly lower than that of OE1-1. The application of 3-OH MAME increased more EPS I production in the phcB-deleted mutant and strain OE1-1 than in the lecM mutant. Thus, QS-dependent produced LecM contributes to the QS signalling pathway. This article is protected by copyright. All rights reserved.

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Name: Molecular plant pathology
ISSN: 1364-3703
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Medical and Biotech [MESH] Definitions

A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.

A species of Ralstonia previously classed in the genera PSEUDOMONAS and BURKHOLDERIA. It is an important plant pathogen.

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The type species in the genus RALSTONIA. It is often found in the hospital ward as a contaminant of antiseptic and disinfectant solutions.

A specific mannose-binding member of the collectin family of lectins. It binds to carbohydrate groups on invading pathogens and plays a key role in the MANNOSE-BINDING LECTIN COMPLEMENT PATHWAY.

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