Advertisement

Topics

Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity.

07:00 EST 9th November 2018 | BioPortfolio

Summary of "Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity."

Fluorescent nucleobase analogues (FBAs) have many desirable features in comparison to extrinsic fluorescent labels, but they are yet to find application in ultrasensitive detection. Many of the disadvantages of FBAs arise from their short excitation wavelengths (often in the ultraviolet), making two-photon excitation a potentially attractive approach. Pentacyclic adenine (pA) is a recently developed FBA that has an exceptionally high two-photon brightness. We have studied the two-photon-excited fluorescence properties of pA and how they are affected by incorporation in DNA. We find that pA is more photostable under two-photon excitation than via resonant absorption. When incorporated in an oligonucleotide, pA has a high two-photon cross section and emission quantum yield, varying with sequence context, resulting in the highest reported brightness for such a probe. The use of a two-photon microscope with ultrafast excitation and pulse shaping has allowed the detection of pA-containing oligonucleotides in solution with a limit of detection of ∼5 molecules, demonstrating that practical single-molecule detection of FBAs is now within reach.

Affiliation

Journal Details

This article was published in the following journal.

Name: Physical chemistry chemical physics : PCCP
ISSN: 1463-9084
Pages:

Links

DeepDyve research library

PubMed Articles [10614 Associated PubMed Articles listed on BioPortfolio]

Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation.

Manipulation of neuronal activity using two-photon excitation of azobenzene photoswitches with near-infrared light has been recently demonstrated, but their practical use in neuronal tissue to photost...

Deterministic Free-Space Source of Single Photons Using Rydberg Atoms.

We propose an efficient free-space scheme to create single photons in a well-defined spatiotemporal mode. To that end, we first prepare a single source atom in an excited Rydberg state. The source ato...

Nonlinear Spectroscopy with X-Ray Two-Photon Absorption in Metallic Copper.

X-ray two-photon absorption (TPA) spectrum of metallic copper is measured using a free-electron laser (XFEL). The spectrum differs from that measured by the conventional one-photon absorption (OPA), a...

A mitochondria targetable two-photon excited near-infrared fluorescent probe for imaging of HO in live cells and tissues.

Hydrogen peroxide (HO), a member of small-molecule reactive oxygen species (ROS), plays an important role in physiological and/or pathological process within live systems. Herein, to quantitatively in...

A high dynamic range optical detector for measuring single photons and bright light.

Detecting light is fundamental to all optical experiments and applications. At the single photon level, the quantized nature of light requires specialised detectors, which typically saturate when more...

Clinical Trials [3206 Associated Clinical Trials listed on BioPortfolio]

Randomized Comparison of Proton and Carbon Ion Radiotherapy With Advanced Photon Radiotherapy in Skull Base Meningiomas: The PINOCCHIO Trial.

In PINOCCHIO-Triayl, carbon ion radiotherapy is compared to proton and advanced photon radiotherapy in patients with skull base meningiomas. There will be two treatment arms with photons, ...

Proton Beam Therapy for Chondrosarcoma

The goal of this clinical research study is to learn if proton beam therapy, with or without photon beam radiation therapy, is effective in the treatment of skull base chondrosarcoma. The ...

Translational Development of Photon-counting CT Imaging

Background: Computed Tomography (CT) scans use x-rays to make three-dimensional images of the body. A new type of CT scanner, called a photon counting CT, measures the x-rays differently ...

C-shaped Root Canal Prevalence and Configurations in Egyptians

The aim of this study is to investigate the prevalence and configuration of C-shaped root canal of mandibular first and second molars among members of an Egyptian sub-population using cone...

Safety and Electrical Performances Evaluation of Navigo Leads Equipped With IS4 Connector

The primary objective of this study is to assess the safety and electrical performance of Left ventricular (LV) NAVIGO 4LV leads, pre-shaped (S-shaped&U-shaped: NAVIGO 4LV 2D, NAVIGO 4LV A...

Medical and Biotech [MESH] Definitions

Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.

Use of a pulse of X-rays or fast electrons to generate free radicals for spectroscopic examination.

Evaluation of the contour of the PULSE waves which vary in different parts of the circulation and depend on physiological as well as pathophysiological conditions of the individual.

Tomography using single-photon emitting RADIONUCLIDES to create images that are captured in times corresponding to various points in the cardiac cycle.

Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topic

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...


Searches Linking to this Article