Advertisement

Topics

Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae.

07:00 EST 17th November 2018 | BioPortfolio

Summary of "Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae."

Glutathione has diverse physiological functions, and therefore, the demand for it has increased recently. Currently, industrial mass production of glutathione is performed from D-glucose via fermentation by the budding yeast Saccharomyces cerevisiae. However, use of D-glucose often competes with demands for various other industries, leading to high production costs. To affordably produce glutathione, we aimed to produce high amounts of glutathione from D-glucose and D-xylose, which are the main constituents of lignocellulosic biomass pre-treated with acids. Genetically engineered S. cerevisiae strains that can produce high amounts of glutathione and assimilate D-xylose were constructed and cultured in media containing D-xylose. Among these recombinant strains, a S. cerevisiae GCI (XR/XDH/XK) strain over-expressing γ-glutamylcysteine synthetase, glutathione synthetase, D-xylose reductase, xylitol dehydrogenase, and xylulokinase genes successfully consumed D-xylose in the medium and produced the highest amount of glutathione. When strains were grown in media containing D-glucose and D-xylose, the GCI (XR/XDH/XK) strain showed 4.6-fold higher volumetric glutathione production (mg/L-broth), 2.2-fold higher glutathione content (%), and 2.1-fold higher cell growth (g-cell/L-broth) than the vector control strain of YPH499 (Vector). Furthermore, when recombinant S. cerevisiae strains were grown in medium containing fermentation inhibitory materials, the GCI (XR/XDH/XK) strain produced 5.8- and higher volumetric glutathione, 2.6-fold higher intracellular glutathione, and 2.9-fold higher cell growth than the vector control YPH499 (Vector) strain. The gradual sugar consumption by recombinant S. cerevisiae strains in medium containing D-glucose and D-xylose leads to high yields of glutathione. These results indicate the potential for glutathione production from lignocellulosic materials.

Affiliation

Journal Details

This article was published in the following journal.

Name: Applied microbiology and biotechnology
ISSN: 1432-0614
Pages:

Links

DeepDyve research library

PubMed Articles [11728 Associated PubMed Articles listed on BioPortfolio]

Sustainable PHA Production in Integrated Lignocellulose Biorefineries.

In emerging bioeconomies, the compostable biopolymers polyhydroxyalkanoates (PHAs) are desirable products due to their similarity to petropolymers. While industrial PHA production has been growing rap...

Recent advances in engineering Corynebacterium glutamicum for utilization of hemicellulosic biomass.

Corynebacterium glutamicum has been mainly used for industrial production of amino acids, and in recent years, it has also been successfully engineered to broaden its range of substrate and product pr...

Processing Lignocellulose-Based Composites into an Ultrastrong Structural Material.

Nature lignocellulose has been a significant renewable raw material due to high specific mechanical properties, in comparison to the values of traditional reinforcing fibres. However, the unsatisfacto...

Lignocellulose fractionation into furfural and glucose by AlCl-catalyzed DES/MIBK biphasic pretreatment.

Herein, an efficient DES/MIBK biphasic pretreatment system for preparation of furfural and fermentable glucose from lignocellulose was developed with AlCl as catalysis. The low-cost and renewable DES ...

Development of betaine-based sustainable catalyst for green conversion of carbohydrates and biomass into 5-hydroxymethylfurfural.

This work develops renewable and sustainable betaine-based catalysts (BX), which are derived from betaine sugar industry or ChCl, for the production of 5-hydroxymethylfurfural (HMF) from various carbo...

Clinical Trials [2647 Associated Clinical Trials listed on BioPortfolio]

Study of Glutathione, Vitamin C and Cysteine in Children With Autism and Severe Behavior Problems

This is a double-blind, placebo-controlled crossover study to evaluate the safety and efficacy of glutathione alone or glutathione, vitamin C and NAC treatment in children with autism who ...

The Effects of Obesity on Glutathione Levels in Patients With Chronic Periodontitis Before and After Periodontal Therapy

Obesity may affect periodontal health by inducing gingival oxidative damage through increased production in circulating reactive oxygen species (ROS). Previous studies have reported decrea...

Effects of Glutathione on Immunity in Individuals Training for a Marathon

The aim of this study is to determine the effects of glutathione supplement on the immune cell response and symptomatology of upper respiratory health, and antioxidant capacity in healthy ...

Review of French Cases of Glutathione Synthetase Deficiency

The glutathione synthetase deficiency, inborn error of metabolism of autosomal recessive inheritance, is a rare disease (70 patients described in the world). The outcome of these patients ...

The Production of Reactive Oxygen Species in Response to Glutathione Supplementation and Acute Exercise

Objectives: The research focus of the study is the production of reactive oxygen species (ROS) in patients with type 2 diabetes (T2D) in response to glutathione (GSH) supplementation and i...

Medical and Biotech [MESH] Definitions

Catalyzes the oxidation of GLUTATHIONE to GLUTATHIONE DISULFIDE in the presence of NADP+. Deficiency in the enzyme is associated with HEMOLYTIC ANEMIA. Formerly listed as EC 1.6.4.2.

An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC 1.11.1.9.

A glutathione transferase that catalyzes the conjugation of electrophilic substrates to GLUTATHIONE. This enzyme has been shown to provide cellular protection against redox-mediated damage by FREE RADICALS.

SUGARS containing an amino group. GLYCOSYLATION of other compounds with these amino sugars results in AMINOGLYCOSIDES.

A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Recombinant DNA
Recombinant DNA is the formation of a novel DNA sequence by the formation of two DNA strands. These are taken from two different organisms. These recombinant DNA molecules can be made with recombinant DNA technology. The procedure is to cut the DNA of ...

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...


Searches Linking to this Article