Advertisement

Topics

Pomegranate seed in diet, affects sperm parameters of cloned goats following freezing-thawing.

07:00 EST 12th November 2018 | BioPortfolio

Summary of "Pomegranate seed in diet, affects sperm parameters of cloned goats following freezing-thawing."

Semen cryopreservation is affected by individual differences and use of cloned animal from the same source is the main tool to eliminate genetic variation. Pomegranate seed (PS) contains fatty acids and phenolic compounds which have antioxidant properties. Essential fatty acids and antioxidants are vital for production of healthy sperm by improving sperm membrane integrity and protecting sperm from oxidative stress. In this study, the effect of dietary supplementation of PS on some blood metabolites and sperm parameters following freezing-thawing of semen of cloned goats were investigated. 5-6 years cloned male goats (N = 9, 50 ± 2 kg) were randomly assigned to three different isocaloric and isonitogenous diet groups: supplemented with 0 (control), 30 and 60% (g/100 g of barley) replaced with PS for a total of 9 weeks. Sperm collection was carried out within 10-14 weeks. Semen samples were diluted whit cryo-protectant and frozen in liquid nitrogen. Sperm parameters, reactive oxygen species (ROS) as well as, ability to induce fertilization were evaluated following freezing/thawing. According to the results of our study, treatment with PS induce higher plasma cholesterol production compared to control group at 8th week. However, testosterone and MDA (malondialdehyde) level of blood plasma were not significantly affected by PS treatment. In comparison to control group, PS supplementation significantly improved total sperm motility and viability in both 30 and 60% PS groups and reduced ROS production. Cleavage rate and developmental competency to blastocyst stage were similar to fresh sperm. In conclusion, dietary supplementation with PS can improve sperm motility and viability following freezing-thawing and maintain developmental competency.

Affiliation

Journal Details

This article was published in the following journal.

Name: Theriogenology
ISSN: 1879-3231
Pages: 203-209

Links

DeepDyve research library

PubMed Articles [11258 Associated PubMed Articles listed on BioPortfolio]

Highly methylated Xist in SCNT embryos was retained in deceased cloned female goats.

X (inactive)-specific transcript (Xist) is crucial in murine cloned embryo development, but its role in cloned goats remains unknown. Therefore, in this study we examined the expression and methylatio...

Characterization of a NAC transcription factor involved in the regulation of pomegranate seed hardness (Punica granatum L.).

The pomegranate, Punica granatum L., which has been cultivated since antiquity, is known to be a superfruit, possessing an array of functional anti-oxidants and various other health benefits. The hard...

Addition of pomegranate juice (Punica granatum) in tris-based extender improves post-thaw quality, motion dynamics and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa.

The aim of the present study was to determine the protective effects of pomegranate juice in tris-based extender on semen parameters, computer-assisted sperm analysis (CASA) motion characteristics and...

The effect of the staining technique on morphological and morphometric parameters of boar sperm.

Sperm morphology and morphometry are important parameters in predicting fertility. Sperm are considered to be normal if the shape and size of the head, midpiece and tail fall within the classification...

Mammary cancer risk and serum lipid profile of rats supplemented with pomegranate seed oil and bitter melon extract.

The objective of the present study was to determine the influence of dietary supplementation with pomegranate seed oil (PSO) and/or an aqueous extract of dried bitter melon fruits (BME) on breast canc...

Clinical Trials [6687 Associated Clinical Trials listed on BioPortfolio]

Macrobiotic Diet and Flax Seed: Effects on Estrogens, Phytoestrogens, & Fibrinolytic Factors

This study will assess whether alternative, high phytoestrogen dietary interventions result in favorable effects on biological parameters that have been associated with hormone-dependent c...

What is the Best Sperm Source and Way of Sperm Selection in Cases With Abnormal sORP Levels on the Day of ICSI?

Does the level of statistic oxidation reduction potential (sORP) affects the choice of sperm source or sperm selection method used during ICSI.

Antioxidant Effects of Pomegranate Juice Versus Placebo in Adults With Type 2 Diabetes Mellitus

This is a research study designed to look at how pomegranate juice (or a placebo juice) affects the body. Participants will be asked to drink 8 oz. of juice daily for 12 weeks. Biomarkers,...

The Effect of Licorice Root Extract and Pomegranate Juice on Atherosclerotic Parameters in Diabetic Patients

The study will check whether taking 100 mg of licorice root extract or 250 cc of pomegranate juice daily by men with diabetes mellitus who are non-smokers and already treated with statins ...

The Beneficial Effects of Pomegranate on Hearing of Patients Without Hemodialysis

Pomegranate has anti-oxidative capacity. It might reduce symptoms of Alzheimer's disease, Drug-induced hepatitis, and might prevent deterioration of cardiovascular diseases and cancer prog...

Medical and Biotech [MESH] Definitions

The state of failure to complete the process of GERMINATION of an intact viable seed, due to intrinsic physical, molecular, genetic, or physiological parameters under otherwise favourable prevailing conditions.

The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.

An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).

Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.

A diet that contains limited amounts of CARBOHYDRATES. This is in distinction to a regular DIET.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Stress
Stress is caused by your perception of situations around you and then the reaction of your body to them. The automatic stress response to unexpected events is known as 'fight or flight'. Discovered by Walter Cannon in 1932, it is the release of h...


Searches Linking to this Article