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Characterization of Caenorhabditis elegans Nucleosome Assembly Protein 1 Uncovers the Role of Acidic Tails in Histone Binding.

07:00 EST 6th December 2018 | BioPortfolio

Summary of "Characterization of Caenorhabditis elegans Nucleosome Assembly Protein 1 Uncovers the Role of Acidic Tails in Histone Binding."

Nucleosome assembly proteins (Naps) influence chromatin dynamics by directly binding to histones. Here we provide a comprehensive structural and biochemical analysis of a Nap protein from Caenorhabditis elegans (CeNap1). CeNap1 naturally lacks the acidic N-terminal tail and has a short C-terminal tail compared to many other Nap proteins. Comparison with full length and a tail-less constructs of Saccharomyces cerevisiae Nap1 (ScNap1) uncovers the role of these tails in self-association, histone-binding, and competing H2A-H2B from DNA. We find that the presence of tails influences the stoichiometry of H2A-H2B-binding and is required to compete interactions between H2A-H2B and DNA. The absolute stoichiometry of the Nap protein and H2A-H2B complex is 2:1 or 2:2, with only a very small population of higher-order oligomers occurring at 150 mM NaCl. We also show that H3-H4 binds differently than H2A-H2B, and that a (H3-H4) tetramer can simultaneously bind two Nap protein homodimers.

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This article was published in the following journal.

Name: Biochemistry
ISSN: 1520-4995
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Medical and Biotech [MESH] Definitions

A histone chaperone that facilitates nucleosome assembly by mediating the formation of the histone octamer and its transfer to DNA.

Proteins from the nematode species CAENORHABDITIS ELEGANS. The proteins from this species are the subject of scientific interest in the area of multicellular organism MORPHOGENESIS.

A genus of small free-living nematodes. Two species, CAENORHABDITIS ELEGANS and C. briggsae are much used in studies of genetics, development, aging, muscle chemistry, and neuroanatomy.

A family of structurally related proteins that were originally discovered for their role in cell-cycle regulation in CAENORHABDITIS ELEGANS. They play important roles in regulation of the CELL CYCLE and as components of UBIQUITIN-PROTEIN LIGASES.

A species of nematode that is widely used in biological, biochemical, and genetic studies.

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