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Trichodysplasia spinulosa associated polyomavirus (TSPyV), a newly identified polyomavirus has been implicated as a causative agent of trychodysplasia spinulosa (TS), a rare proliferative skin disease in severly immunocompromised hosts. The diagnosis using monoclonal antibodies (mAbs) has been provided as a promising tool as it offers high specificity towards specific antigen. Till there is no good mAb available for diagnosis of TS disease. In this study we developed and characterized the mAbs specific for VP1 of TSPyV. We used wheat germ cell-free synthesized VP1 protein of TSPyV for immunizing BALB/c mice to generate hybridomas. Screening of the resultant hybridoma clones we selected five strongly positive clones producing mAbs reactive to the TSPyV-VP1 antigen. Epitope mapping and bioinformatic analysis showed that these mAbs recognized epitopes located within highly conserved C-terminal region of all clinical isolates of TSPyV-VP1. Further all the mAbs were highly effective for immunofluorescense and immunoprecipitation analysis. Among five mAbs, three clones did not exhibit any cross reactivity to VP1 of other related polyomaviruses. In addition, one of our mAbs (#14) provided immunohistochemical staining of skin tissue of TS disease. It can be concluded that among this panel of anti-VP1 antibodies, three mAbs might provide a useful set of tools for the study of TSPyV infection as well as the development of specific diagnosis.
This article was published in the following journal.
Name: Microbiology and immunology
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Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.
Antibodies from non-human species whose protein sequences have been modified to make them nearly identical with human antibodies. If the constant region and part of the variable region are replaced, they are called humanized. If only the constant region is modified they are called chimeric. INN names for humanized antibodies end in -zumab.
Antibodies obtained from a single clone of cells grown in mice or rats.
Combinations of diagnostic or therapeutic substances linked with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; or ANTIGENS. Often the diagnostic or therapeutic substance is a radionuclide. These conjugates are useful tools for specific targeting of DRUGS and RADIOISOTOPES in the CHEMOTHERAPY and RADIOIMMUNOTHERAPY of certain cancers.
Conditions characterized by the presence of M protein (Monoclonal protein) in serum or urine without clinical manifestations of plasma cell dyscrasia.
Monoclonal antibodies MAbs
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