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Nanogels enable efficient miRNA delivery and target gene downregulation in transfection-resistant multiple myeloma cells.

07:00 EST 20th December 2018 | BioPortfolio

Summary of "Nanogels enable efficient miRNA delivery and target gene downregulation in transfection-resistant multiple myeloma cells."

Multiple myeloma is a common plasma cell-derived hematologic neoplasm. While the delivery of growth inhibiting miRNA to multiple myeloma cells would be a promising strategy to evaluate treatment options, most multiple myeloma cells are transfection-resistant with established methods. Non-viral nanoparticulate transfection systems are particularly promising in this context, but so far struggle with transfection and knockdown efficiency. Here we present poly(glycidol) based nanogels with covalently bound cell penetration peptide TAT (transactivator of transcription from HIV). TAT facilitated a varying internalization efficiency of the nanogels depending on the cell line. The positively charged peptide also served as complexation agent for miRNA and enabled covalent binding of the TAT/miR-34a complex in the nanogels. These TAT/miRNA loaded nanogels delivered and released miR-34a with high efficiency into OPM-2 multiple myeloma cells that are known as transfection-resistant. Delivery resulted in efficient downregulation of known target genes such as Notch1, Hey1, Hes6 and Hes1. Thus, these nanogel constructs offer a new tool to enhance gene delivery into multiple myeloma cells with immediate value in cancer research.

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This article was published in the following journal.

Name: Biomacromolecules
ISSN: 1526-4602
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