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L-Fucose production by engineered Escherichia coli.

07:00 EST 29th December 2018 | BioPortfolio

Summary of "L-Fucose production by engineered Escherichia coli."

L-fucose (6-deoxy-L-galactose) is a major constituent of glycans and glycolipids in mammals. Fucosylation of glycans can confer unique functional properties, and may be an economic way to manufacture of L-fucose. Researches can extract L-fucose directly from brown algae, or by enzymatic hydrolysis of L-fucose-rich microbial exopolysaccharides. However, these L-fucose production methods are not economical or scalable for various applications. We engineered an Escherichia coli strain to produce L-fucose. Specifically, we modified the strain genome to eliminate endogenous L-fucose metabolism, produce 2-fucosyllactose (2-FL), and to liberate L-fucose from 2-FL. This E. coli strain produced 16.7 g/L of L-fucose with a productivity of 0.1 g/L·h in a fed-batch fermentation. This study presents an efficient one-pot biosynthesis strategy to produce a monomeric form of L-fucose by microbial fermentation, making large-scale industrial production of L-fucose feasible. This article is protected by copyright. All rights reserved.

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Name: Biotechnology and bioengineering
ISSN: 1097-0290
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