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In this study, a novel and efficient aptasensor based on immobilization of thiol terminated prostate specific antigen (PSA) binding DNA aptamer onto Au nanoparticles/fullerene C-chitosan-ionic liquid/multiwalled carbon nanotubes/screen printed carbon electrode has been fabricated for ultrasensitive aptasensing of biomarker PSA. Formation of PSA-aptamer complex caused a variation in electrochemical impedance spectroscopic (EIS) and differential pulse voltammetric (DPV) responses of the aptasensor which enabled us to aptasensing of the PSA by EIS and DPV methods. Morphology and electrochemical properties of the fabricated aptasensor were examined by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS. The aptasensor was successfully applied to the determination of PSA by EIS and DPV in the range of 1-200 pg mL with a limit of detection (LOD) of 0.5 pg mL and 2.5-90 ng mL with a LOD of 1.5 ng mL, respectively. This aptasensor exhibited outstanding anti-interference ability towards co-existing molecules with good stability, sensitivity, repeatability and reproducibility. Practical application of the aptasensor was examined with analysis of the PSA levels in serum samples obtained from patients with prostate cancer using both the aptasensor and a reference method. The results revealed the proposed system to be a promising candidate for clinical analysis of PSA.
This article was published in the following journal.
Name: International journal of biological macromolecules
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Use of cutaneous electrochemical conductance for preclinical screening of small-caliber nerve fibers
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A scanning probe microscopy technique that uses an ultramicroelectrode as the scanning probe that simultaneously records changes in electrochemical potential as it scans thereby creating topographical images with localized electrochemical information.
The practice of prescribing or using a drug outside the scope of the drug's official approved label as designated by a regulatory agency concerning the treatment of a particular disease or condition.
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.
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Benign Prostatic Hyperplasia (BPH)
An enlarged prostate (benign prostatic enlargement (BPE) or benign prostatic hyperplasia (BPH)) is common in men after the age of about 50. Having an enlarged prostate does not mean you have cancer. In some cases, an enlarged prostate can cause the ...
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