Combining high wavenumber and fingerprint Raman spectroscopy for the detection of prostate cancer during radical prostatectomy.

08:00 EDT 15th August 2018 | BioPortfolio

Summary of "Combining high wavenumber and fingerprint Raman spectroscopy for the detection of prostate cancer during radical prostatectomy."

For prostate cancer (PCa) patients, radical prostatectomy (complete removal of the prostate) is the only curative surgical option. To date, there is no clinical technique allowing for real-time assessment of surgical margins to minimize the extent of residual cancer. Here, we present a tissue interrogation technique using a dual excitation wavelength Raman spectroscopy system capable of sequentially acquiring fingerprint (FP) and high wavenumber (HWN) Raman spectra. Results demonstrate the ability of the system to detect PCa in post-prostatectomy specimens. In total, 477 Raman spectra were collected from 18 human prostate slices. Each area measured with Raman spectroscopy was characterized as either normal or cancer based on histopathological analyses, and each spectrum was classified based on supervised learning using support vector machines (SVMs). Based on receiver operating characteristic (ROC) analysis, FP (area under the curve [AUC] = 0.89) had slightly superior cancer detection capabilities compared with HWN (AUC = 0.86). Optimal performance resulted from combining the spectral information from FP and HWN (AUC = 0.91), suggesting that the use of these two spectral regions may provide complementary molecular information for PCa detection. The use of leave-one-(spectrum)-out (LOO) or leave-one-patient-out (LOPO) cross-validation produced similar classification results when combining FP with HWN. Our findings suggest that the application of machine learning using multiple data points from the same patient does not result in biases necessarily impacting the reliability of the classification models.


Journal Details

This article was published in the following journal.

Name: Biomedical optics express
ISSN: 2156-7085
Pages: 4294-4305


DeepDyve research library

PubMed Articles [21690 Associated PubMed Articles listed on BioPortfolio]

High-Throughput Cell Imaging and Classification by Narrowband and Low-Spectral-Resolution Raman Microscopy.

We investigated the use of narrowband Raman spectra for rapid label-free molecular imaging aimed at cell classification using principal component regression and linear discriminant analysis. In the cl...

Raman Optical Activity and Raman spectroscopy of carbohydrates in solution.

This comprehensive study on selected 14 carbohydrates in water solution is an extension of previously published one focused only on solid state analysis. Here, Raman spectroscopy was used as a dedicat...

Development and first in-human use of a Raman spectroscopy guidance system integrated with a brain biopsy needle.

Navigation-guided brain biopsies are the standard of care for diagnosis of several brain pathologies. However, imprecise targeting and tissue heterogeneity often hinder obtaining high-quality tissue s...

SERS based monitoring of toluene vapors at ambient and elevated temperatures by using a ruffled silver nanolayer as a substrate.

The authors describe a Surface enhanced Raman spectroscopy (SERS)-based method for the detection of gaseous toluene at different temperature regimes using 3D ruffled silver SERS substrates and a comme...

Broadband Fourier-transform coherent Raman spectroscopy with an ytterbium fiber laser.

We demonstrate a Fourier transform (FT) coherent anti-Stokes Raman scattering (CARS) spectroscopy system based on fiber technology with ultra-broad spectral coverage and high-sensitivity. A femtosecon...

Clinical Trials [9034 Associated Clinical Trials listed on BioPortfolio]

Confocal Raman Spectroscopy: in Vivo Measurement of Physiological Skin Parameters

The purpose of this study is to I. determine several skin parameters, for example natural moisturizing factor (NMF) and cholesterol, with Raman spectroscopy in vivo and II. compare...

Multimodal Spectroscopy (MMS) for in Vivo Noninvasive Assessment of Skin

The goal of this research program is to develop a simple, noninvasive diagnostic device for assessing skin pathology without the need for a biopsy. The device being studied is a single sys...

Non-invasive Glucose Measurement Using Laser Technology

This study is to evaluate the capability of Raman spectroscopy as a non-invasive method for monitoring concentrations of glucose by illuminating a small spot on the skin, collecting and an...

Raman Probe for In-vivo Diagnostics (During Oesophageal) Endoscopy

To develop and endoscopic Raman spectroscopy probe for delivery down and channel in an endoscope to make near instant assessments of the condition of the oesophagus without the need for ex...

Non-Invasive Detection of Tissue Oxygen Deprivation in Premature Infants With Patent Ductus Arteriosus.

The proposed research evaluates tissue oxygenation (StO2) as measured by resonance raman spectroscopy (RRS) in premature infants with and without patent ductus arteriosus (PDA). This is a ...

Medical and Biotech [MESH] Definitions

Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.

Magnetic resonance spectroscopy involving detection of changes in the resonance of carbon-13 nuclei in organic molecules.

Proteins secreted by the prostate gland. The major secretory proteins from the human prostate gland include PROSTATE-SPECIFIC ANTIGEN, prostate-specific acid phosphatase, prostate-specific membrane antigen, and prostate-specific protein-94.

Tissue ablation of the PROSTATE performed by ultrasound from a transducer placed in the RECTUM. The procedure is used to treat prostate cancer (PROSTATIC NEOPLASMS) and benign prostatic hypertrophy (PROSTATIC HYPERPLASIA).

A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.

Quick Search


DeepDyve research library

Relevant Topics

  Bladder Cancer Brain Cancer Breast Cancer Cancer Cervical Cancer Colorectal Head & Neck Cancers Hodgkin Lymphoma Leukemia Lung Cancer Melanoma Myeloma Ovarian Cancer Pancreatic Cancer ...

Prostate Cancer
Prostate cancer (cancer de prostata) Prostate cancer (cancer de prostata) is a form of cancer that develops in the prostate, a gland in the male reproductive system. Most prostate cancers are slow growing; however, there are cases of aggressive prostat...

Searches Linking to this Article