Characterization of DNPH-coated microreactor chip for analysis of trace carbonyls with application for breath analysis.

07:00 EST 6th January 2019 | BioPortfolio

Summary of "Characterization of DNPH-coated microreactor chip for analysis of trace carbonyls with application for breath analysis."

The analysis of trace carbonyls including aldehydes and ketones is important for monitoring environmental air quality, determining toxicity of aerosol of electronic cigarette, and detecting diseases by breath analysis. This work reports investigation of a single microreactor chip with HClO-acidified DNPH coating for capture and analysis of carbonyls in air and exhaled breath. Three aldehydes and three ketones were spiked into one liter synthetic air in Tedlar bags serving as gaseous carbonyl standard for characterization of capture efficiency (CE). The HClO-acidified DNPH showed higher CE of carbonyls than conventionally-used acid including HPO and HSO acidified DNPH under the microreactor conditions. The microreactor conditions including HClO to DNPH molar ratio, DNPH to carbonyls molar ratio, and gaseous sample flow rate through the microreactor were studied in detail and thereby optimized. Under the optimized conditions, 100% of CEs for aldehydes and above 80% for ketones were obtained. The microreactor chips were applied to determine acetone concentration in exhaled breath.


Journal Details

This article was published in the following journal.

Name: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Pages: 58-63


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