Calcium Imaging of Store-Operated Calcium (Ca) Entry (SOCE) in HEK293 Cells Using Fura-2.

07:00 EST 1st January 2019 | BioPortfolio

Summary of "Calcium Imaging of Store-Operated Calcium (Ca) Entry (SOCE) in HEK293 Cells Using Fura-2."

The store-operated calcium (Ca) entry (SOCE) pathway is an essential Ca signaling pathway in non-excitable cells that serve many physiological functions. SOCE is mediated through the plasma membrane (PM) protein, Orai1, and the endoplasmic reticulum protein, stromal interaction molecule 1 (STIM1). One of the most well-established methods to study SOCE is using the Ca-sensing dye, fura-2. Here we describe a detailed protocol on how to use fura-2 to study Ca signaling from SOCE in human embryonic kidney (HEK) cells.


Journal Details

This article was published in the following journal.

Name: Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Pages: 163-172


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