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Fuel-responsive allosteric DNA-based aptamers for the transient release of ATP and cocaine.

07:00 EST 4th February 2019 | BioPortfolio

Summary of "Fuel-responsive allosteric DNA-based aptamers for the transient release of ATP and cocaine."

Allostery is generally considered as a thermodynamic equilibrium phenomenon. In contrast to this, we show here that this mechanism offers a key strategy to rationally design out-of-equilibrium synthetic devices. We demonstrate this by engineering allosteric DNA-based nanodevices for the transient load and release of small organic molecules. To demonstrate the versatility and generality of our approach we have employed two model DNA-based aptamers that bind ATP and cocaine through a target-induced conformational change. We have rationally re-engineered these aptamers so that their affinity towards their specific target is controlled by a DNA sequence acting as an allosteric inhibitor. The use of an enzyme that specifically cleaves the inhibitor only when it is bound to the aptamer generates a transient allosteric control that leads to the temporal release of ATP or cocaine from the aptamers. Our approach confirms how the programmability and predictability of nucleic acids make synthetic DNA/RNA the perfect candidate material to re-engineer synthetic receptors that can undergo chemical fuel-triggered release of different kinds of small molecule cargoes (ATP and cocaine) and to rationally design non-equilibrium systems. Moreover, our study illustrates the potential of transient allosteric regulation as a tool to control the functions of synthetic dissipative devices.

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This article was published in the following journal.

Name: Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
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