Labelling strategy and membrane characterization of marine bacteria Vibrio splendidus by in vivoH NMR.

07:00 EST 2nd February 2019 | BioPortfolio

Summary of "Labelling strategy and membrane characterization of marine bacteria Vibrio splendidus by in vivoH NMR."

Vibrio splendidus is a marine bacterium often considered as a threat in aquaculture hatcheries where it is responsible for mass mortality events, notably of bivalves' larvae. This bacterium is highly adapted to dynamic salty ecosystems where it has become an opportunistic and resistant species. To characterize their membranes as a first and necessary step toward studying bacterial interactions with diverse molecules, we established a labelling protocol for in vivoH solid-state nuclear magnetic resonance (SS-NMR) analysis of V. splendidus. H SS-NMR is a useful tool to study the organization and dynamics of phospholipids at the molecular level, and its application to intact bacteria is further advantageous as it allows probing acyl chains in their natural environment and study membrane interactions. In this study, we showed that V. splendidus can be labelled using deuterated palmitic acid, and demonstrated the importance of surfactant choice in the labelling protocol. Moreover, we assessed the impact of lipid deuteration on the general fitness of the bacteria, as well as the saturated-to-unsaturated fatty acid chains ratio and its impact on the membrane properties. We further characterize the evolution of V. splendidus membrane fluidity during different growth stages and relate it to fatty acid chain composition. Our results show larger membrane fluidity during the stationary growth phase compared to the exponential growth phase under labelling conditions - an information to take into account for future in vivo SS-NMR studies. Our lipid deuteration protocol optimized for V. splendidus is likely applicable other microorganisms for in vivo NMR studies.


Journal Details

This article was published in the following journal.

Name: Biochimica et biophysica acta. Biomembranes
ISSN: 1879-2642


DeepDyve research library

PubMed Articles [16343 Associated PubMed Articles listed on BioPortfolio]

Hemolymph C1qDC promotes the phagocytosis of oyster Crassostrea gigas hemocytes by interacting with the membrane receptor β-integrin.

Phagocytosis constitutes a conserved cellular process for multicellular animals to ingest or engulf other cells or particles, which is facilitated by the use of opsonins to bind foreign particles and ...

Periplasmic solute-binding proteins: Structure classification and chitooligosaccharide recognition.

Periplasmic solute-binding proteins (SBPs) serve as molecular shuttles that assist the transport of small solutes from the outer membrane to the inner membrane of all Gram-negative bacteria. Based on ...

Cell-cell communication, chemotaxis, and recruitment in Vibrio parahaemolyticus.

Motile bacteria are proficient at finding optimal environments for colonization. Often, they use chemotaxis to sense nutrient availability and dangerous concentrations of toxic chemicals. For many bac...

A theoretical model to estimate inactivation effects of OH radicals on marine Vibrio sp. in bubble-shock interaction.

A theoretical model for estimating inactivation effects on marine Vibrio sp. is developed from the viewpoint of the chemical action of the OH radicals induced by interaction of bubbles with shock wave...

Does Ralstonia eutropha, rich in poly-β hydroxybutyrate (PHB), protect blue mussel larvae against pathogenic vibrios?

The natural amorphous polymer poly-β-hydroxybutyrate (PHB-A: lyophilized Ralstonia eutropha containing 75% PHB) was used as a biological agent to control bacterial pathogens of blue mussel (Mytilus e...

Clinical Trials [4071 Associated Clinical Trials listed on BioPortfolio]

Marine Protein Hydrolysate as Dietary Supplement in Elderly Part I

The aim of this study is to investigate the potential effect of a marine protein hydrolysate (MPH) supplement before a meal on postprandial glucose tolerance in healthy subjects, to achiev...

The Long-term Effect of Marine Omega-3 Fatty Acid Supplementation in Renal Transplantation

This is a long-term intervention study on the effects of marine n-3 PUFAs in renal transplantation. Our hypothesis is that patients treated with marine n-3 PUFA supplementation will have l...

Direct Molecular Characterization of Bacteria From ICU

Investigators aim to directly investigate the molecular properties of bacteria from tracheal and urinary samples routinely taken in intensive care units (ICU) patients.

Evaluation of Calcium and Vitamin D Supplementation During Marine Corps Training

The primary objective of this randomized, double-blind, placebo-controlled trial is to determine the efficacy and effectiveness of Ca and vitamin D supplementation provided daily throughou...

Marine Protein Hydrolysate as Dietary Supplement in Elderly

The potential use of marine protein hydrolysates (MPH) as a supplement with similar or better health benefits than a regular white fish meal, can be regarded both cost-effective, environme...

Medical and Biotech [MESH] Definitions

A species of gram-negative, halophilic bacteria, in the genus VIBRIO. It is considered part of normal marine flora and commonly associated with ear infections and superficial wounds exposed to contaminated water sources.

A strain of the VIBRIO CHOLERAE bacteria belonging to serogroup non-O1, infecting humans and other PRIMATES. It is related to VIBRIO CHOLERAE O1, but causes a disease less severe than CHOLERA. Eating raw shellfish contaminated with the bacteria results in GASTROENTERITIS.

A species of bacteria found in the marine environment, sea foods, and the feces of patients with acute enteritis.

A species of gram-negative bacteria in the genus VIBRIO, isolated from SHELLFISH, as well as from human diarrheal stools and ear infections.

A species of gram-negative bacteria in the genus VIBRIO, the causative agent of cold water vibriosis, a HEMORRHAGIC SEPTICEMIA of ATLANTIC SALMON.

Quick Search


DeepDyve research library

Searches Linking to this Article