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A mutant strain of Pyropia yezoensis, strain E, was isolated from the free living conchocelis of a pure strain (NA) treated with ethyl methane sulfonate. The incremental quantities of young strain E blades were higher than those of NA after 14 d of cultivation, indicating that young blades of mutant strain E released more archeospores. The mean length and weight of large E blades were both over three times greater than those of NA after 4 weeks of cultivation. The photosynthetic parameters (Fv/Fm, Y[I], Y[II], and O evolution rate) and pigment contents (including phycoerythrin and phycocyanin) of strain E blades were higher than those of NA (p<0.05). The cellular respiratory rate of strain E blades was lower than that of NA (p<0.05). In order to investigate the causes of changes in strain E blades, total RNA in strain E and NA blades were sequenced using the Illumina Hiseq platform. Compared with NA, 1,549 unigenes were selected in strain E including 657 up-regulated and 892 down-regulated genes. According to the physiology measurement and DEGs analysis, cell respiration in strain E might decrease, while anabolic-like photosynthesis and protein biosynthesis might increase compared with NA. This means substance accumulation might be greater than decomposition in strain E. This might explain why strain E blades showed improved growth compared with NA. In addition, several genes related to stress resistance were up-regulated in strain E indicating that strain E might have a higher stress resistance. The sequencing dataset may be conducive to Pyropia yezoensis molecular breeding research. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Journal of phycology
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