Advertisement

Topics

Generic Neutravidin Biosensor for Simultaneous Multiplex Detection of MicroRNAs via Electrochemically Encoded Responsive Nanolabels.

07:00 EST 7th February 2019 | BioPortfolio

Summary of "Generic Neutravidin Biosensor for Simultaneous Multiplex Detection of MicroRNAs via Electrochemically Encoded Responsive Nanolabels."

Current electrochemical biosensors for multiple miRNAs require tedious immobilization of various nucleic acid probes. Here, we demonstrate an innovative approach using a generic neutravidin biosensor combined with electrochemically encoded responsive nanolabels for facile and simultaneous multiplexed detection of miRNA-21 and miRNA-141. The selectivity of the biosensor arises from the intrinsic properties of the electrochemically encoded responsive nanolabels, comprising biotinylated molecular beacons (biotin-MB) and metal nanoparticles (metal-NPs). The procedure is a simple one-pot assay, where the targeted miRNA causes the opening of biotin-MB followed by capturing of the biotin-MB-metal-NPs by the neutravidin biosensor and simultaneous detection of the captured metal-NPs by stripping square-wave voltammetry (SSWV). The multiplexed detection of miRNA-21 and miRNA-141 is achieved by differentiation of the electrochemical signature (i.e., the peak current) for the different metal-NP labels. The biosensor delivers simultaneous detection of miRNAs with a linear range of 0.5-1000 pM for miRNA-21 and a limit of detection of 0.3 pM (3σ/sensitivity,  n = 3), and a range of 50-1000 pM for miRNA-141, with a limit of detection of 10 pM. Furthermore, we demonstrate multiplexed detection of miRNA-21 and miRNA-141 in a spiked serum sample.

Affiliation

Journal Details

This article was published in the following journal.

Name: ACS sensors
ISSN: 2379-3694
Pages:

Links

DeepDyve research library

PubMed Articles [8573 Associated PubMed Articles listed on BioPortfolio]

Multiplex Touchdown PCR assay to enhance specificity and sensitivity for concurrent detection of four foodborne pathogens in raw milk.

The aim of this study was to develop a multiplex touchdown PCR (multiplex TD-PCR) for rapid and simultaneous detection of four major foodborne pathogens to avoid mispriming and unwanted production dur...

Cell-based biosensor with dual signal outputs for simultaneous quantification of phenylacetic acid and phenylethylamine.

Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosens...

Hierarchical assembled nanomaterial paper based analytical devices for simultaneously electrochemical detection of microRNAs.

Paper based biosensor devices have the benefit of easy-use, low-cost and environmental friendly for point-of-care diagnostic application. In this research, we report a novel form of paper based biosen...

Towards a dual in-line electrochemical biosensor for the determination of glucose and hydrogen peroxide.

Herein, we report the development of a sensitive and selective dual mode electrochemical platform for the detection of glucose and HO. The platform is based on tungsten and gold microwire electrodes d...

Multiplex-PCR for diagnosis of bacterial meningitis.

Considering the great lethality and sequels caused by meningitis, rapid diagnosis and prompt treatment initiation have a great impact on patient outcome. Here, we developed a multiplex-PCR for simulta...

Clinical Trials [2700 Associated Clinical Trials listed on BioPortfolio]

Detection of Gastrointestinal Bleeding in Intensive Care Patients Via Biosensor Watch

The purpose of this study to improve methods of monitoring and diagnosing gastrointestinal bleeding via the E4 wristband, a biosensor watch.

Non Invasive Detection of Cardiac Allograft Rejection by Circulating microRNAs

The purpose of this study is to evaluate the level of expression of 4 circulating microRNAs in the serum using RT-PCR. A pilote study with cardiac transplant patients has shown that expres...

OstiSense Biosensor in Bruxism Reduction - A Clinical Study

The overall objective of the clinical studies with the OstiSense biosensor tool will be to evaluate whether the use of the OstiSense biosensor tool significantly reduces the number of slee...

Characterization of Epilepsy Patients BEEP 2b

Some epilepsy patients are described as GB when they have worsened seizures or side effects related to switching between brand name and generic, or between generic antiepileptic drug (AED)...

Early Molecular Detection Technique Coupled With Urinary Test of Infectious Agents Responsible of Children CAP

Community-Acquired Pneumonia (CAP) of children are a recurrent pathology with multiple severity scores. The etiology is never really identified, and the initial treatment is always based o...

Medical and Biotech [MESH] Definitions

The simultaneous use of multiple laboratory procedures for the detection of various diseases. These are usually performed on groups of people.

A disorder characterized by multiple, wide spread cutaneous cysts that often become inflamed and rupture. It is caused by the same mutations in the gene coding for KRT-17 that are causative mutations for Pachyonychia congenita, Type 2. Natal teeth involvement is sometimes associated with steatocystoma multiplex.

Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.

The simultaneous, or near simultaneous, transference of heart and lungs from one human or animal to another.

Simultaneous infection of a host organism by two or more pathogens. In virology, coinfection commonly refers to simultaneous infection of a single cell by two or more different viruses.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Generics Drugs
A generic drug (generic drugs, short: generics) is a drug defined as "a drug product that is comparable to brand/reference listed drug product in dosage form, strength, route of administration, quality and performance characteristics, and intended u...

Drug Discovery
Clinical Approvals Clinical Trials Drug Approvals Drug Delivery Drug Discovery Generics Drugs Prescription Drugs In the fields of medicine, biotechnology and pharmacology, drug discovery is the process by which drugs are dis...


Searches Linking to this Article