Encapsulated human mesenchymal stem cells (eMSCs) as a novel anti-cancer agent targeting breast cancer stem cells: development of 3D primed therapeutic MSCs.

07:00 EST 5th February 2019 | BioPortfolio

Summary of "Encapsulated human mesenchymal stem cells (eMSCs) as a novel anti-cancer agent targeting breast cancer stem cells: development of 3D primed therapeutic MSCs."

Breast cancer is a leading cause of mortality in women across the globe. The major reason for its recurrence and high mortality is due to the presence of a drug refractory and self-renewing population of cells, the cancer stem cells (CSCs). Mesenchymal stem cells (MSCs) have recently emerged as a promising cell-based therapeutic agent for the treatment of different cancer types. However, the anti-tumor effect of MSCs has been indicated to be substantially reduced by their in vivo tumor-trophic migration property and direct cell-to-cell integration with tumor stromal elements. To address this drawback, the present study uses a biomaterial, sodium alginate, for the encapsulation of MSCs from the perinatal tissue, Wharton's jelly (WJMSCs) into microbeads, to study the effect of WJMSCs beads on breast CSCs derived from two breast cancer cell lines, MDA-MB-231 and MCF7. Encapsulation with sodium alginate facilitated the prevention of direct cell-to-cell interaction and these microbeads provided a three-dimensional (3D) microenvironment for the encapsulated WJMSCs (eWJMSCs). Compared to two dimensional (2D) culture, eWJMSC increased proliferation of WJMSCs with an increase in pluripotency genes, epithelial to mesenchymal transition (EMT), immune-modulation, and angiogenesis. Interestingly, the tumor invasion suppressor protein E-cadherin was highly expressed in eWJMSCs as shown by Western blot analysis. In addition, eWJMSCs had an increased secretion of anti-inflammatory cytokines VEGF, TGF-β, TNF-α, IFN-γ, IL-10 and -6, and IL-3β when compared to 2D culture. Treatment of CSCs with eWJMSCs reduced cell viability, inhibited migration, and exerted an anti-angiogenic effect. eWJMSCs treatment of CSCs increased caspase 3/7 activity, nitric oxide production, and reactive oxygen species production, suggesting its anti-tumorigenic activity. Gene expression analysis revealed that CSCs treated with eWJMSCs had a downregulation of pro-proliferation markers, drug transporters, epithelial-mesenchymal transition-associated markers, and angiogenesis related genes. The mode of anti-proliferative action of WJMSCs beads was possibly through inhibition of the Wnt/β-catenin signaling pathway as indicated by the upregulation of the Wnt antagonists sFRP4 and DKK1. These data suggest that alginate-encapsulated WJMSCs could be an efficient cell contact-free system for developing stem cell-based therapies for CSCs.


Journal Details

This article was published in the following journal.

Name: The international journal of biochemistry & cell biology
ISSN: 1878-5875


DeepDyve research library

PubMed Articles [29160 Associated PubMed Articles listed on BioPortfolio]

Descemet's Membrane Biomimetic Microtopography Differentiates Human Mesenchymal Stem Cells Into Corneal Endothelial-Like Cells.

Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. Corneal transplantation is currently the only therapy for severe corne...

Blended Nanostructured Degradable Mesh with Endometrial Mesenchymal Stem Cells Promotes Tissue Integration and Anti-Inflammatory Response in Vivo for Pelvic Floor Application.

The current urogynaecological clinical meshes trigger unfavourable foreign body response which leads to graft failure in the long term. To overcome the present challenge we applied a tissue engineerin...

Human dental pulp stem cells regulate allogeneic NK cells' function via induction of anti-inflammatory purinergic signalling in activated NK cells.

Mesenchymal stem cells (MSCs) could regulate the function of various immune cells. It remains unclear whether MSCs additionally possess immunostimulatory properties. We investigated the impact of huma...

miR-21 Contributes to Human Amniotic Membrane-Derived Mesenchymal Stem Cell Growth and Human Amniotic Membrane-Derived Mesenchymal Stem Cell-Induced Immunoregulation.

Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are considered a new and favorable source of stem cells for cell replacement-based therapy. Some microRNAs (miRNAs) have been reported...

EphA2-positive human umbilical cord-derived mesenchymal stem cells exert anti-fibrosis and immunomodulatory activities via secretion of prostaglandin E2.

Previous study has demonstrated that EphA2 is a biomarker of mesenchymal stem cells (MSCs) from human placenta or umbilical cord and is able to distinguish MSCs from fibroblasts. In this study, we fur...

Clinical Trials [14985 Associated Clinical Trials listed on BioPortfolio]

Dysfunctions of Human Muscle Stem Cells in Sepsis

The dysfunction of muscle stem cells after sepsis is demonstrated in mice. It is also observed that mesenchymal stem cells have a positive effect on muscle stem cells in this animal model....

Encapsulated Mesenchymal Stem Cells for Dental Pulp Regeneration.

To compare the dental survival in a period of 2 years of mature permanent teeth with apical lesion following the administration of encapsulated Mesenchymal Stem Cells under a regenerative ...

The Safety/Efficacy of Human Umbilical Cord Mesenchymal Stem Cells Therapy for Patients With Osteoarthritis

The purpose of this study is verify the efficacy and safety of Human Umbilical Cord Mesenchymal Stem Cells (UC-MSC) therapy for patients with Osteoarthritis, and in addition, provide basis...

Human Mesenchymal Stem Cells For Bronchopulmonary Dysplasia

This study is an open-label, single-center, dose escalation study to evaluate of safety and efficacy of human umbilical cord -derived mesenchymal stem cells (hUC-MSCs) in premature infants...

Clinical Study of Human Umbilical Cord Mesenchymal Stem Cells in the Treatment of Decompensated Hepatitis b Cirrhosis

1. Evaluation the safety of using human umbilical mesenchymal stem cells to treat patients with hepatitis B cirrhosis. 2. Observe the curative effect of patients with hepatitis B c...

Medical and Biotech [MESH] Definitions

Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.

Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).

Cells that can develop into distinct mesenchymal tissue such as BONE; TENDONS; MUSCLES; ADIPOSE TISSUE; CARTILAGE; NERVE TISSUE; and BLOOD and BLOOD VESSELS.

A type of PLURIPOTENT STEM CELLS derived from early stage human embryos, up to and including the BLASTOCYST stage.

Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from:

Quick Search


DeepDyve research library

Relevant Topics

Stem Cells
Track and monitor developments in stem cell research and commercial development.  Follow the tabs above to read the latest global news, research, clinical trials on stem cells and follow companies active in the stem cell industry.  BioPort...

Cancer Disease
Cancer is not just one disease but many diseases. There are more than 100 different types of cancer. Most cancers are named for the organ or type of cell in which they start - for example, cancer that begins in the colon is called colon cancer; cancer th...

Women's Health
Women's Health - key topics include breast cancer, pregnancy, menopause, stroke Follow and track Women's Health News on BioPortfolio: Women's Health News RSS Women'...

Searches Linking to this Article