Advertisement

Topics

Determination of metabolites of the UV filter 2-ethylhexyl salicylate in human urine by online-SPE-LC-MS/MS.

07:00 EST 14th February 2019 | BioPortfolio

Summary of "Determination of metabolites of the UV filter 2-ethylhexyl salicylate in human urine by online-SPE-LC-MS/MS."

The UV filter 2‑ethylhexyl salicylate (EHS) is widely used in sunscreens and other personal care products (PCP). EHS has been detected in a variety of environmental matrices. However, data on the internal EHS exposure in humans is not available, due to the lack of exposure biomarkers and analytical methods for their determination. Here, we report a method for the determination of three oxidative EHS metabolites in human urine: 2‑ethyl‑5‑hydroxyhexyl 2‑hydroxybenzoate (5OH-EHS), 2‑ethyl‑5‑oxohexyl 2‑hydroxybenzoate (5oxo-EHS), and 5‑(((2‑hydroxybenzoyl)oxy)methyl)heptanoic acid (5cx-EPS). Urine samples are incubated with β‑glucuronidase and analyzed by liquid chromatography-electrospray ionization-triple quadrupole-tandem mass spectrometry, coupled with online sample clean-up and analyte enrichment using turbulent flow chromatography (online-SPE-LC-MS/MS). Quantification is performed by stable isotope dilution analysis, using deuterium-labeled standards of each of the three metabolites. The described method is precise (coefficient of variation <5% within-series and interday), accurate (mean relative recoveries between 96% and 105%), and sensitive, with limits of quantification (LOQ) of 0.01 μg/L (5cx-EPS), 0.05 μg/L (5OH-EHS), and 0.15 μg/L (5oxo-EHS). After dermal application of an EHS containing sunscreen to a human volunteer, we were able to quantify all three metabolites in urine samples collected post application, showing clear elimination kinetics. In spot urine samples from the general population (n = 35) we were able to quantify EHS biomarkers in 91% of all samples, with highest concentrations in individuals (n = 11) who stated use of PCPs containing UV filters within 5 days prior to sampling. We will apply the method for investigating human EHS metabolism and in future human biomonitoring studies for EHS exposure and risk assessment.

Affiliation

Journal Details

This article was published in the following journal.

Name: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Pages: 59-66

Links

DeepDyve research library

PubMed Articles [16058 Associated PubMed Articles listed on BioPortfolio]

Determination of human urinary metabolites of the plasticizer di(2-ethylhexyl) adipate (DEHA) by online-SPE-HPLC-MS/MS.

Di(2-ethylhexyl) adipate (DEHA) is a plasticizer and phthalate substitute used in various consumer products. Relevant population exposures have to be assumed. In this study we describe the determinati...

Urinary Metabolites of the UV filter 2-Ethylhexyl Salicylate as Biomarkers of Exposure in Humans.

The UV filter 2-ethylhexyl salicylate (EHS) is used in sunscreens and other personal care products worldwide and has been found in a variety of environmental media. We aimed to provide human toxicokin...

Allergic contact dermatitis from ethylhexyl salicylate with possible cross-reactivity to benzyl salicylate.

Ethylhexyl salicylate (CAS no. 118-60-5), also known as octyl salicylate or octisalate, is classified as UV B absorber according to the EU cosmetic regulation (COSING), and permitted in a concentratio...

Fate of UV filter Ethylhexyl methoxycinnamate in rat model and human urine: Metabolism, exposure and demographic associations.

Ethylhexyl methoxycinnamate (EHMC) is one of the most frequently used UV filters in sunscreens and other cosmetic products. Its ubiquitous presence in various environmental matrices and its endocrine ...

Comparison of the Detection Windows of Heroin Metabolites in Human Urine Using Online SPE and LC-MS/MS: Importance of Morphine-3-Glucuronide.

Heroin abuse is a serious problem that endangers human health and affects social stability. Though often being used as confirmation of heroin use, 6-monoacetylmorphine (6-MAM) has limitations due to i...

Clinical Trials [6594 Associated Clinical Trials listed on BioPortfolio]

Longvida Curcumin Human Pharmacokinetics Study

The primary objective of the study is to characterize the curcumin metabolites of 2 different capsule formulations of curcumin relative to unformulated Curcuma longa extract of Rhizomes in...

Pre-analytical Sample Handling Conditions and Their Effects on the Human Urine Metabolome

This study evaluates how urine handling conditions can affect the concentrations of ~1,000 urine metabolites and attempts to quantify the degree of attenuation and/or potential bias in epi...

Recovery Filter Study (EVEREST)

This study was designed to assess the safety of retrieval of the Bard Recovery® G2® Filter System. The G2 filter is an FDA-cleared device for inferior vena caval interruption in patients...

Vena Cava Filter U.S. Clinical Trial

The investigation is designed to verify that clinical use of the vena cava filter does not raise new questions of safety or effectiveness compared to currently-marketed permanent filters.

Filter Ventilation

The goal of this study is to determine the biomarkers of exposure and harm as well as the pattern of product purchase when ventilated vs. unventilated cigarettes are available in the marke...

Medical and Biotech [MESH] Definitions

A non-steroidal anti-inflammatory agent that is less effective than equal doses of ASPIRIN in relieving pain and reducing fever. However, individuals who are hypersensitive to ASPIRIN may tolerate sodium salicylate. In general, this salicylate produces the same adverse reactions as ASPIRIN, but there is less occult gastrointestinal bleeding. (From AMA Drug Evaluations Annual, 1992, p120)

A protein extract of human menopausal urine in which LUTEINIZING HORMONE has been partially or completely removed. Urofollitropin represents FOLLICLE STIMULATING HORMONE from the urine.

A computerized biomedical bibliographic storage and retrieval system operated by the NATIONAL LIBRARY OF MEDICINE. MEDLARS stands for Medical Literature Analysis and Retrieval System, which was first introduced in 1964 and evolved into an online system in 1971 called MEDLINE (MEDLARS Online). As other online databases were developed, MEDLARS became the name of the entire NLM information system while MEDLINE became the name of the premier database. MEDLARS was used to produce the former printed Cumulated Index Medicus, and the printed monthly Index Medicus, until that publication ceased in December 2004.

A means of identifying the age of an animal or human through tooth examination.

Supporting content or information, such as animation, datasets, multimedia files, video, movies, audio files, text files, or software, which is submitted for publication in an online journal or an online edition of a journal. This information may be referenced in the text of the article with a link to the supplementary data provided. CATALOG: do not use

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Urology
Benign Prostatic Hyperplasia (BPH) Erectile Dysfunction Urology Urology is the branch of medicine concerned with the urinary tract and diseases that affect it. Examples include urethritis, urethrostenosis and incontinence. Urology is a su...

Multiple Sclerosis MS
Multiple sclerosis (MS) is the most common disabling neurological condition affecting 100,000 young adults in the UK. The condition results from autoimmune damage to myelin, causing interference in nerve signaling. Symptoms experienced depend on the pa...


Searches Linking to this Article