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Celosin A (CA), a natural compound isolated from Celosia argentea L., has been shown significant hepatoprotective effect on AHNP-induced liver injury. This study described a rapid and sensitive ultra high pressure liquid chromatography-tandem mass spectrometry (UHPLC- MS/MS) assay for determining of CA in rat plasma. Methanol-mediated precipitation was used for sample pretreatment. Chromatographic separation was achieved on a T3 column with gradient elution using water and acetonitrile as mobile phase. Determination was obtained using an electrospray ionization source in negative selected reaction monitoring (SRM) mode at the transitions of m/z 793.3→m/z 661.2 and m/z 955.6→m/z 793.2 for CA and IS, respectively. The assay was linear over the concentration range 0.25-2500 ng/mL (r > 0.995) with the lowest limit of quantification (LLOQ) of 0.25 ng/mL. The intra- and inter-day precisions (RSD, %) ranged 1.65-9.84% and 2.46-13.49%, respectively, while accuracy (RR, %) ranged from 96.21% to 99.45%, respectively. The recovery ranged from 95.09% to 102.22% and the matrix effect from 98.29% to 100.13%. The analyte was stable under tested storage conditions. The method has been successfully applied to a preclinical pharmacokinetic study in rats after a single intravenous (2 mg/kg) and oral (50 mg/kg) administration. The oral bioavailability of CA was about 1.94%, in addition, there was no difference between male and female rats. As we know, it is the first time of the use of UHPLC-MS/MS method for determination of CA concentration in rat plasma and for evaluation of its pharmacokinetic behaviors.
This article was published in the following journal.
Name: Biomedical chromatography : BMC
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