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One-Pot Production of RNA Nanoparticles via Automated Processing and Self Assembly.

08:00 EDT 19th March 2019 | BioPortfolio

Summary of "One-Pot Production of RNA Nanoparticles via Automated Processing and Self Assembly."

Only 1.5% of the human genome codes for protein. More and more evidence shows that the other 98.5% of the so-called junk DNA actually codes for small or large non-coding RNAs that regulate cellular activities. In addition to the milestones of chemical and protein drugs, it is predicted that a third milestone in drug development is RNA as drugs or drugs targeting to RNA (Guo et al., Adv Drug Deliv Rev. 2014 66:74). Currently the yield and cost of RNA nanoparticle or RNA drug production has been a barricade for the advancement of the RNA field in both research and clinical translation due to the multiple tedious manufacturing steps. For example, with a 98.5% of incorporation efficiency of chemical synthesis of a 100-nucleotide, RNA oligos will result in 78% contamination of the aborted bi-products. Here we report the one-pot production of RNA nanoparticles via automated processing and self-assembly. The continuous production of RNA by rolling circle transcription (RCT) using circular dsDNA template is coupled with the self-cleaving ribozyme encoded in the concatemeric RNA transcripts. Production was monitored in real time. Automatic production of RNA fragments enabled their assembly either in situ or via one-pot co-transcription to obtain RNA nanoparticles of desired motifs and functionalities from bottom-up assembly of multiple RNA fragments. In combination with the RNA nanoparticle construction process, a purification method using large scale electrophoresis column was also developed.

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This article was published in the following journal.

Name: ACS nano
ISSN: 1936-086X
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