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Transcriptomic mechanism of a phytohormone 6-benzylaminopurine (6-BAP) stimulating lipid and DHA synthesis in Aurantiochytrium sp.

08:00 EDT 22nd March 2019 | BioPortfolio

Summary of "Transcriptomic mechanism of a phytohormone 6-benzylaminopurine (6-BAP) stimulating lipid and DHA synthesis in Aurantiochytrium sp."

The phytohormone 6-benzylaminopurine (6-BAP) significantly improves lipid synthesis of oleaginous microorganisms with the great potential applied in lipid production. In the current study, the lipid and DHA productions in oleaginous Aurantiochytrium sp. were found to be improved by 48.7 % and 55.3 %, respectively, induced by 6-BAP treatments. Then, using high-throughput RNA-seq technology, the overall de novo assembly of the cDNA sequence data generated 53,871 unigenes, and 15,902 of these were annotated in at least one database. The comparative transcriptomic profiles of cells with and without 6-BAP treatments revealed that a total of 717 were differently expressed genes (DE), with 472 upregulated and 245 downregulated. Further annotation and categorization indicated that some DE genes were involved in pathways crucial to lipid and DHA productions, such as fatty acid synthesis, central carbon metabolism, transcriptional factor, signal transduction, and mevalonate pathway. A regulation mode of 6-BAP, in turn, perception and transduction of 6-BAP signal, transcription factor, expression regulations of the downstream genes and metabolic changes, respectively, was put forward for the first time in present study. This research illuminates the transcriptomic mechanism of phytohormone stimulating lipid and DHA production in the oleaginous microorganism and provides the potential targets modified using genetic engineering for improving lipid and DHA productivity.

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This article was published in the following journal.

Name: Journal of agricultural and food chemistry
ISSN: 1520-5118
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