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Coculture of Endothelial Cells with Human Pluripotent Stem Cell-Derived Cardiac Progenitors Reveals a Differentiation Stage-Specific Enhancement of Cardiomyocyte Maturation.

08:00 EDT 30th March 2019 | BioPortfolio

Summary of "Coculture of Endothelial Cells with Human Pluripotent Stem Cell-Derived Cardiac Progenitors Reveals a Differentiation Stage-Specific Enhancement of Cardiomyocyte Maturation."

Cardiomyocytes (CMs) generated from human pluripotent stem cells (hPSCs) are immature in their structure and function, limiting their potential in disease modeling, drug screening, and cardiac cellular therapies. Prior studies have demonstrated that coculture of hPSC-derived CMs with other cardiac cell types, including endothelial cells (ECs), can accelerate CM maturation. To address whether the CM differentiation stage at which ECs are introduced affects CM maturation, we cocultured hPSC-derived ECs with hPSC-derived cardiac progenitor cells (CPCs) and CMs, and analyzed molecular and functional attributes of maturation. ECs had a more significant effect on acceleration of maturation when cocultured with CPCs than with CMs. EC coculture with CPCs increased CM size, expression of sarcomere and ion channel genes and proteins, the presence of intracellular membranous extensions, and chronotropic response compared to monoculture. Maturation was accelerated with increasing
EC:
CPC ratio. This study demonstrates that EC incorporation at the CPC stage of CM differentiation expedites CM maturation, leading to cells that may be better suited for in vitro and in vivo applications of hPSC-derived CMs. This article is protected by copyright. All rights reserved.

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Journal Details

This article was published in the following journal.

Name: Biotechnology journal
ISSN: 1860-7314
Pages: e1800725

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