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Broadening the Scope of Enforced ATP Wasting as a Tool for Metabolic Engineering in Escherichia coli.

08:00 EDT 30th March 2019 | BioPortfolio

Summary of "Broadening the Scope of Enforced ATP Wasting as a Tool for Metabolic Engineering in Escherichia coli."

The targeted increase of cellular ATP turnover (enforced ATP wasting) has recently been recognized as a promising tool for metabolic engineering when product synthesis is coupled with net ATP formation. The goal of the present study was to further examine and to further develop the concept of enforced ATP wasting and to broaden its scope for potential applications. In particular, considering the fermentation products synthesized by E. coli under anaerobic conditions as a proxy for target chemical(s), we (i) developed a new genetic module for dynamic and gradual induction of the F -part of the ATPase and found that (ii) induction of the ATPase leads to higher metabolic activity and increased product formation in E. coli also under anaerobic conditions and that (iii) ATP wasting significantly increases substrate uptake and productivity of growth-arrested cells which is vital for its use in two-stage processes. To the best of our knowledge, the glucose uptake rate of 6.49 mmol/gCDW/h achieved with enforced ATP wasting is the highest value reported for non-growing E. coli cells. In summary, we showed that enforced ATP wasting can be used to improve yield and titer (in growth-coupled processes) as well as volumetric productivity (in two-stage processes) depending on which of the performance measures is more crucial for the process and product of interest. This article is protected by copyright. All rights reserved.

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This article was published in the following journal.

Name: Biotechnology journal
ISSN: 1860-7314
Pages: e1800438

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