Track topics on Twitter Track topics that are important to you
The targeted increase of cellular ATP turnover (enforced ATP wasting) has recently been recognized as a promising tool for metabolic engineering when product synthesis is coupled with net ATP formation. The goal of the present study was to further examine and to further develop the concept of enforced ATP wasting and to broaden its scope for potential applications. In particular, considering the fermentation products synthesized by E. coli under anaerobic conditions as a proxy for target chemical(s), we (i) developed a new genetic module for dynamic and gradual induction of the F -part of the ATPase and found that (ii) induction of the ATPase leads to higher metabolic activity and increased product formation in E. coli also under anaerobic conditions and that (iii) ATP wasting significantly increases substrate uptake and productivity of growth-arrested cells which is vital for its use in two-stage processes. To the best of our knowledge, the glucose uptake rate of 6.49 mmol/gCDW/h achieved with enforced ATP wasting is the highest value reported for non-growing E. coli cells. In summary, we showed that enforced ATP wasting can be used to improve yield and titer (in growth-coupled processes) as well as volumetric productivity (in two-stage processes) depending on which of the performance measures is more crucial for the process and product of interest. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Biotechnology journal
Transcriptional biosensors allow screening, selection, or dynamic regulation of metabolic pathways, and are, therefore, an enabling technology for faster prototyping of metabolic engineering and susta...
5-aminolevulinic acid (ALA), an important precursor of tetrapyrroles, has various applications in medicine and agriculture fields. Several methods have been adopted to enhance ALA synthesis in our pre...
Bioethanol has been considered as a potentially renewable energy source, and metabolic engineering plays an important role in the production of biofuels. As an efficient ethanol-producing bacterium, Z...
Escherichia coli (E. coli) is a promising platform for expression of full-length antibodies owing to its several advantages as a production host (fast growth, well characterized genetics, low manufact...
In silico kinetic modeling is an essential tool for rationally designing metabolically engineered organisms based on a system-level understanding of their regulatory mechanisms. However, an estimation...
To allow patients with HIV-associated wasting to receive thalidomide treatment at 1 of 2 doses. To gain safety and efficacy data from a broader base of patients with HIV-associated wasting...
The purpose of this study is to see if giving nandrolone decanoate (a hormonal drug) will cause weight gain in HIV-positive women who have HIV-associated weight loss (wasting). Wasting ha...
Despite the characterization of many aetiologic genetic changes. The specific causative factors in the development of sporadic colorectal cancer remain unclear. This study was performed to...
The objective of this study is to evaluate the imaging capabilities and image analysis performance of the PhotoniCare TOMi Scope in pediatric patients undergoing tympanostomy tube placemen...
A cross validation study comparing an international acknowledged assessment tool "Endoscopy Training System" (ETS) and movement analysis of the colonoscope based on raw data from coils wit...
Methods and techniques used to modify or select cells and develop conditions for growing cells for biosynthetic production of molecules (METABOLIC ENGINEERING), for generation of tissue structures and organs in vitro (TISSUE ENGINEERING), or for other BIOENGINEERING research objectives.
Strains of ESCHERICHIA COLI that are a subgroup of SHIGA-TOXIGENIC ESCHERICHIA COLI. They cause non-bloody and bloody DIARRHEA; HEMOLYTIC UREMIC SYNDROME; and hemorrhagic COLITIS. An important member of this subgroup is ESCHERICHIA COLI O157-H7.
A non-aqueous co-solvent that serves as tool to study protein folding. It is also used in various pharmaceutical, chemical and engineering applications.
Strains of Escherichia coli that possess virulence traits which allow them to invade, colonize, and induce disease in tissues outside of the GASTROINTESTINAL TRACT. They are a cause of URINARY TRACT INFECTIONS (UROPATHOGENIC ESCHERICHIA COLI); neonatal MENINGITIS; SEPSIS; PNEUMONIA; and SURGICAL WOUND INFECTION.
Instruments for the visual examination of interior structures related to NEUROSURGICAL PROCEDURES. There are two broad categories of neuroendoscopes, the rigid scope and the flexible scope, for various types of procedures.