Enhanced Saccharification and Fermentation of Rice Straw by Reducing the Concentration of Phenolic Compounds Using an Immobilized Enzyme Cocktail.

08:00 EDT 30th March 2019 | BioPortfolio

Summary of "Enhanced Saccharification and Fermentation of Rice Straw by Reducing the Concentration of Phenolic Compounds Using an Immobilized Enzyme Cocktail."

The production of bioethanol from rice straw can contribute to the rural economy and provide clean fuel in a sustainable manner. However, phenolic compounds, which are mostly produced during acid pretreatment of biomass, act as inhibitors of fermenting microorganisms. Laccase is well known for its ability to oxidize lignin and phenolic compounds derived from lignocellulosic biomass. In the present study, an immobilized enzyme cocktail containing laccase was evaluated in regards to its ability to enhance the saccharification and fermentation processes by reducing the amount of phenolic compounds produced. Magnetic Fe O nanoparticles modified with (3-aminopropyl) triethoxysilane-glutaraldehyde exhibited higher immobilization yield and immobilization efficiency in comparison to nanoparticles modified only with glutaraldehyde. Saccharification of rice straw pre-treated with dilute acid with the laccase-supplemented immobilized enzyme cocktail reduced phenolic compounds by 73.8%, resulting in a saccharification yield of 84.6%. In addition, improved yeast performance was noted during the fermentation process, resulting in a 78.3% conversion of sugar into ethanol with an ethanol productivity of 0.478 g/L/h. To the best of our knowledge, this is the first description of the use of an immobilized enzyme cocktail comprised of Celluclast 1.5L, β-glucosidase, and laccase for the production of bioethanol from rice straw. This study details a potential approach to producing biofuels from agricultural biomass, the applicability of which can be improved through up-scaling. This article is protected by copyright. All rights reserved.


Journal Details

This article was published in the following journal.

Name: Biotechnology journal
ISSN: 1860-7314
Pages: e1800468


DeepDyve research library

PubMed Articles [14085 Associated PubMed Articles listed on BioPortfolio]

Evaluation of inhibitory effect and feasible utilization of dilute acid-pretreated rice straws on succinate production by metabolically engineered Escherichia coli AS1600a.

This work demonstrated a pioneer work in the pre-treatment of rice straw by phosphoric acid (HPO) for succinate production. The optimized pre-treatment condition of rice straw was at 121 °C for 30...

Enhanced butyric acid production using mixed biomass of brown algae and rice straw by Clostridium tyrobutyricum ATCC25755.

A brown alga Saccharina japonica and rice straw are attractive feedstock for microbial butyric acid production. However, inefficient fermentation of mannitol (a dominant component in S. japonica) and ...

Evaluating novel fungal secretomes for efficient saccharification and fermentation of composite sugars derived from hydrolysate and molasses into ethanol.

This paper evaluates the ability of secretome from two thermotolerant fungal strains (Aspergillus terreus 9DR and Achaetomium strumarium 10DR) for boosting the hydrolytic efficiency of benchmark cellu...

Isolation and physicochemical characterization of different lignin streams generated during the second-generation ethanol production process.

Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simult...

A comparative account of glucose yields and bioethanol production from separate and simultaneous saccharification and fermentation processes at high solids loading with variable PEG concentration.

A process strategy to aid in optimal enzymatic hydrolysis through the addition of polyethylene glycol (PEG6000) was tested for separate hydrolysis and fermentation (SHF) and simultaneous saccharificat...

Clinical Trials [5354 Associated Clinical Trials listed on BioPortfolio]

Red Rice Yeast in Low-density Lipoprotein (LDL) Lowering: an Efficacy Study

Red yeast rice is a source of active compounds in reducing LDL levels with practically no side effects. Molval Fort is a natural product available in the Lebanese market with a combination...

Bioavailability of Phenolic Compounds in Bread

The consumption of wholemeal cereals has been associated with the reduction of several chronic diseases. The mechanisms behind these protective effects may be linked, besides dietary fiber...

Effect of a Specific Phenolic Compound on Blood Pressure

The study's main objective is to investigate the effects of acute consumption of a preparation containing 100 mg of a specific phenolic compound (patent pending, P201531587) on systolic BP...

Investigation of Acute Mood and Cognitive Performance Effects of Beverages Containing Phenolics in Healthy Adults

An acute, randomized, placebo controlled, double blinded crossover study in health subjects. Participants will complete 4 intervention arms in random order consisting of a placebo control ...

Pilot Feasibility of Rice Bran Supplementation in Nicaraguan Children

The purpose is to assess feasibility of rice bran consumption in weaning children and collect pilot data on gut microbiome and metabolome modulation with rice bran intake for diarrheal pre...

Medical and Biotech [MESH] Definitions

The anamorphic form of the fungus MAGNAPORTHE GRISEA and the most important pathogen of rice worldwide. It causes rice blast which prevents maturation of the rice grains.

OIL extracted from the hard outer brown layer of rice bran after the husk has been removed.

A zinc-containing enzyme which oxidizes primary and secondary alcohols or hemiacetals in the presence of NAD. In alcoholic fermentation, it catalyzes the final step of reducing an aldehyde to an alcohol in the presence of NADH and hydrogen.

A genus of DNA plant viruses in the family CAULIMOVIRIDAE. The sole species, Rice tungro bacilliform virus, shows 25% identity with BADNAVIRUS. It is transmitted by leafhoppers with the assistance of RICE TUNGRO SPHERICAL VIRUS.

A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)

Quick Search


DeepDyve research library

Relevant Topics

Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Searches Linking to this Article