A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for the Marine Scuticociliates.

08:00 EDT 30th March 2019 | BioPortfolio

Summary of "A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for the Marine Scuticociliates."

Pseudocohnilembus persalinus is a free-living marine scuticociliate that, as a new model organism, has been used in a wide variety of studies. However, long-term laboratory maintenance for this species is mainly achieved by sub-culture that requires rigorous culture environments and, too often, cultures of the organism die out for a variety of reasons. Successful transport of viable cultures also poses problems for researchers. The present study describes a simple and rapid protocol for long-term cryopreservation of P. persalinus. The effects of physiological states of individuals before freezing, the type and concentration of cryoprotectant, and optimal temperatures for freezing and thawing were assessed. A cryopreservation protocol, using a mixture of 30% glycerol and 70% concentrated P. persalinus cell culture, incorporating rate-controlled freezing at -80 °C before liquid nitrogen storage, maintained a high recovery efficiency after eight weeks of storage. These results suggest that broader application of this protocol to build a cryopreserved marine protozoa culture bank for biological studies may be possible. This article is protected by copyright. All rights reserved.


Journal Details

This article was published in the following journal.

Name: The Journal of eukaryotic microbiology
ISSN: 1550-7408


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