Determination of Tartrazine, Lutein, Capsanthin, Canthaxanthin and β-Carotene in Animal-Derived Foods and Feeds by HPLC Method.

08:00 EDT 30th March 2019 | BioPortfolio

Summary of "Determination of Tartrazine, Lutein, Capsanthin, Canthaxanthin and β-Carotene in Animal-Derived Foods and Feeds by HPLC Method."

Pigments are still widely used in food and feed industry and their resides in food might be harmful to human health due to their side effects. A high performance liquid chromatography (HPLC) method for simultaneous determination of pigments including tartrazine, lutein, capsanthin, canthaxanthin and β-carotene in animal-derived foods (including the muscle and liver of swine, the muscle, liver and skin of chicken and duck, and the muscle of fish) and feeds (swine, chicken and duck) was developed. Lutein, capsanthin, canthaxanthin and β-carotene were extracted with acetonitrile-ethyl acetate by ultrasonication, and tartrazine was extracted with water, followed by defatting with n-hexane and clean-up by solid phase extraction on weak anion exchange cartridges. The quantitation of the five pigments was performed by HPLC with ultraviolet and visible spectrophotometer detection. Chromatographic separations were performed on a C8 column with gradient elution. The mean recoveries of analytes ranged from 80.4 to 92.5%. The intra- and the inter-day variabilities were below 15.0%. This HPLC method was suitable for the routine determination of pigment residues in animal-derived foods and feeds.


Journal Details

This article was published in the following journal.

Name: Journal of chromatographic science
ISSN: 1945-239X


DeepDyve research library

PubMed Articles [10203 Associated PubMed Articles listed on BioPortfolio]

Mechanisms of Transport and Delivery of Vitamin A and Carotenoids to the Retinal Pigment Epithelium.

Vision depends on the delivery of vitamin A (retinol) to the retina. Retinol in blood is bound to retinol-binding protein (RBP). Retinal pigment epithelia (RPE) cells express the RBP receptor, STRA6 t...

Effect of the diets with pumpkin silage and synthetic β-carotene on the carotenoid, immunoglobulin and bioactive protein content and fatty acid composition of colostrum.

Forty Simmental cows were divided into four groups with 10 per group to investigate the effect of a transition diet supplemented with synthetic β-carotene and natural carotenoid-rich feed (pumpkin si...

Enhancing lutein production with mixotrophic cultivation of Chlorella sorokiniana MB-1-M12 using different bioprocess operation strategies.

A lutein-enriched mutant, Chlorella sorokiniana MB-1-M12 was grown mixotrophically for lutein production. The lutein production efficiency of the strain was enhanced via optimizing the operating strat...

Effect of carotenoid class and dose on the larval growth and development of the critically endangered southern corroboree frog.

Dietary carotenoids are expected to improve vertebrate growth and development, though evidence for beneficial effects remains limited. One reason for this might be that few studies have directly compa...

Characterizing the noncovalent binding behavior of tartrazine to lysozyme: A combined spectroscopic and computational analysis.

Tartrazine is a stable water-soluble azo dye widely used as a food additive, which could pose potential threats to humans and the environment. In this paper, we evaluated the response mechanism betwee...

Clinical Trials [2906 Associated Clinical Trials listed on BioPortfolio]

Carotenoid Absorption and Metabolism From Green-leafy Vegetable Matrices

Absorption and metabolism of carotenoids from two types of spinach preparations will be investigated in two study groups, short bowel/ileostomy and healthy, respectively. The study will b...

Dietary Lutein and the Retinopathy of Prematurity

The clinical trial aspect of this study is the provision of a lutein supplement to lactating women and compare the amount of lutein in the milk and blood with lactating women who do not re...

Canthaxanthin Retinopathy: A Long-term Observation

Canthaxanthine is a naturally occurring carotenoid that has been a popular over-the-counter oral artificial tanning agent in Europe, Canada, and Australia since 1979. It is also used at lo...

Lutein for Age-Related Macular Degeneration

This study will examine whether taking the vitamin lutein changes lutein blood levels. Lutein, vitamin C, vitamin E and beta-carotene may be useful in treating the eye disease age-related ...

The STOVITA Study - Bioefficacy of Beta-Carotene in Oil and in a Mixed Diet in Ileostomy Patients

A controlled dietary intervention study will be carried out based on the stable isotope method the investigators have developed to quantify both the absorption of beta-carotene and its bio...

Medical and Biotech [MESH] Definitions

A trans-carotenoid pigment widely distributed in nature. The compound is used as an oral suntanning agent and as a food and drug coloring agent. Oral ingestion of the compound causes canthaxanthin retinopathy.

A carotenoid produced in most carotenogenic organisms. It is one of several sequentially synthesized molecules that are precursors to BETA CAROTENE.

Oxygenated forms of carotenoids. They are usually derived from alpha and beta carotene.

A monooxygenase that catalyzes the conversion of BETA-CAROTENE into two molecules of RETINAL. It was formerly characterized as EC and EC

Persons who avoid consuming animal products or animal-derived substances, such as MEAT; EGGS; or HONEY. Some vegans also refrain from using animals or animal products in any form or for any purpose, such as in CLOTHING or household goods.

Quick Search


DeepDyve research library

Relevant Topics

Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...

Arthritis Fibromyalgia Gout Lupus Rheumatic Rheumatology is the medical specialty concerned with the diagnosis and management of disease involving joints, tendons, muscles, ligaments and associated structures (Oxford Medical Diction...

Searches Linking to this Article