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Pigments are still widely used in food and feed industry and their resides in food might be harmful to human health due to their side effects. A high performance liquid chromatography (HPLC) method for simultaneous determination of pigments including tartrazine, lutein, capsanthin, canthaxanthin and β-carotene in animal-derived foods (including the muscle and liver of swine, the muscle, liver and skin of chicken and duck, and the muscle of fish) and feeds (swine, chicken and duck) was developed. Lutein, capsanthin, canthaxanthin and β-carotene were extracted with acetonitrile-ethyl acetate by ultrasonication, and tartrazine was extracted with water, followed by defatting with n-hexane and clean-up by solid phase extraction on weak anion exchange cartridges. The quantitation of the five pigments was performed by HPLC with ultraviolet and visible spectrophotometer detection. Chromatographic separations were performed on a C8 column with gradient elution. The mean recoveries of analytes ranged from 80.4 to 92.5%. The intra- and the inter-day variabilities were below 15.0%. This HPLC method was suitable for the routine determination of pigment residues in animal-derived foods and feeds.
This article was published in the following journal.
Name: Journal of chromatographic science
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A trans-carotenoid pigment widely distributed in nature. The compound is used as an oral suntanning agent and as a food and drug coloring agent. Oral ingestion of the compound causes canthaxanthin retinopathy.
A carotenoid produced in most carotenogenic organisms. It is one of several sequentially synthesized molecules that are precursors to BETA CAROTENE.
Oxygenated forms of carotenoids. They are usually derived from alpha and beta carotene.
A monooxygenase that catalyzes the conversion of BETA-CAROTENE into two molecules of RETINAL. It was formerly characterized as EC 184.108.40.206 and EC 220.127.116.11.
Persons who avoid consuming animal products or animal-derived substances, such as MEAT; EGGS; or HONEY. Some vegans also refrain from using animals or animal products in any form or for any purpose, such as in CLOTHING or household goods.
Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...
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