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Functional characterization of two phytochelatin synthases in rice (Oryza sativa cv. Milyang 117) that respond to cadmium stress.

08:00 EDT 31st March 2019 | BioPortfolio

Summary of "Functional characterization of two phytochelatin synthases in rice (Oryza sativa cv. Milyang 117) that respond to cadmium stress."

Cadmium (Cd) is one of the most toxic heavy metals and a non-essential element to all organisms, including plants; however, the genes involved in Cd resistance in plants remain poorly characterized. To identify Cd resistance genes in rice, we screened a rice cDNA expression library treated with CdCl using a yeast (Saccharomyces cerevisiae) mutant ycf1 strain (DTY167) and isolated two rice phytochelatin synthases (OsPCS5 and OsPCS15). The genes were strongly induced by Cd treatment and conferred increased resistance to Cd when expressed in the ycf1 mutant strain. In addition, the Cd concentration was 2-fold higher in yeast expressing OsPCS5 and OsPCS15 than in vector-transformed yeast, and OsPCS5 and OsPCS15 localized in the cytoplasm. Arabidopsis thaliana plants overexpressing OsPCS5/-15 paradoxically exhibited increased sensitivity to Cd, suggesting that overexpression of OsPCS5/-15 resulted in toxicity due to excess phytochelatin production in A. thaliana plants. These data indicate that OsPCS5 and OsPCS15 are involved in Cd tolerance, which may be related to the relative abundances of phytochelatins synthesized by these phytochelatin synthases. This article is protected by copyright. All rights reserved.

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Name: Plant biology (Stuttgart, Germany)
ISSN: 1438-8677
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