Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry.

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Summary of "Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry."

This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔH), K, k, and v are determined in a single assay that does not require labeling or immobilization.


Journal Details

This article was published in the following journal.

Name: Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Pages: 241-249


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