Advertisement

Topics

Thermodynamics of Molecular Machines Using Incremental ITC.

07:00 EST 1st January 2019 | BioPortfolio

Summary of "Thermodynamics of Molecular Machines Using Incremental ITC."

Molecular biomachines, such as DNA and RNA polymerases or the ribosome, are fascinating biological assemblies able to swiftly perform repeated and highly regulated tasks, with a remarkable accuracy. Significant advances in structural studies during the past 20 years provided a wealth of information regarding their architecture and considerably contributed to a better understanding of their mechanism of action. However, the three-dimensional structure of a biological nanomachine alone does not provide access to its detailed mechanism of action, even when obtained at atomic resolution. When combined with other biophysical approaches, thermodynamic data, together with kinetic data, are essential for a complete description of any binding interaction, revealing forces driving complex formation and providing insights into mechanisms of action. We have developed an incremental ITC approach that is well-suitable for analysis of biomolecular machines. This strategy allows a dissection of molecular biomachine reactions through successive additions in the ITC cell of consecutive substrates.

Affiliation

Journal Details

This article was published in the following journal.

Name: Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Pages: 129-140

Links

DeepDyve research library

PubMed Articles [7939 Associated PubMed Articles listed on BioPortfolio]

Trajectory and Cycle-Based Thermodynamics and Kinetics of Molecular Machines: The Importance of Microscopic Reversibility.

A molecular machine is a nanoscale device that provides a mechanism for coupling energy from two (or more) processes that in the absence of the machine would be independent of one another. Examples in...

SNAREpin Assembly: Kinetic and Thermodynamic Approaches.

Proteins constantly interact and often form molecular complexes. The dynamics of most biological processes strongly rely on the kinetics and thermodynamics of assembly and disassembly of these complex...

A Molecular Cable Car for Transmembrane Ion Transport.

The controlled transport of molecular and ionic substrates across bilayer membranes is a fundamental task for the operation of living organisms. It is also a highly fascinating and demanding challenge...

On the Universality of Memcomputing Machines.

Universal memcomputing machines (UMMs) represent a novel computational model in which memory (time nonlocality) accomplishes both tasks of storing and processing of information. UMMs have been shown t...

Molecular Simulations of Ion Effects on the Thermodynamics of RNA Folding.

How ions affect RNA folding thermodynamics and kinetics is an important but a vexing problem that remains unsolved. Experiments have shown that the free energy change, $\Delta G(c)$, of RNA upon foldi...

Clinical Trials [1540 Associated Clinical Trials listed on BioPortfolio]

Spectral-domain Optical Coherence Tomography of the Eye

An increasing number of clinical studies on SD-OCT of ocular pathologies and potential new clinical applications has recently been published in the peer-reviewed literature. However, the s...

Comparison of Gas Consumption From Two Different Anesthesia Machines

Low flow anesthesia (LFA) is used less than desirable, partly because one has considered that there might be danger of underdosing of anesthesia gas and thus risk of awareness under anesth...

Nuclear Myosin VI - a Therapeutic Target in Breast Cancer

Gene expression, the transfer of the genetic code into cellular proteins is one of the most fundamental processes in living cells. This process is orchestrated by protein-based molecular m...

Altering the Availability of Healthier vs. Less Healthy Items in Vending Machines

Background: While there is some evidence that increasing the range of healthier foods and drinks and/or decreasing the range of less healthy options may increase healthier choices, more wo...

Refinement and Assessment of New Magnetic Resonance Imaging (MRI) Technologies for Neurological Exams

This study is being done to assess new Magnetic Resonance Imaging (MRI) technologies such as new hardware or software designed to improve MRI examinations of the brain, spine, and head & n...

Medical and Biotech [MESH] Definitions

Machines that add moisture to the air.

A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)

Methods of creating machines and devices.

The application of molecular biology to the answering of epidemiological questions. The examination of patterns of changes in DNA to implicate particular carcinogens and the use of molecular markers to predict which individuals are at highest risk for a disease are common examples.

Evolution at the molecular level of DNA sequences and proteins. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...


Searches Linking to this Article