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An efficient non-enzymatic electrochemical sensor for hydrogen peroxide (HO) was constructed by modifying a glassy carbon electrode (GCE) with a nanocomposite prepared from cobalt nanoparticle (CoNP) and tungsten carbide (WC). The nanocomposite was prepared at low temperature through a simple technique. Its crystal structure, surface morphology and elemental composition were investigated via X-ray diffraction, transmission electron microscopy and X-ray photoelectron spectroscopy. The results showed the composite to be uniformly distributed and that the CoNP are well attached to the surface of the flake-like WC. Electrochemical studies show that the modified GCE has an improved electrocatalytic activity toward the reduction of HO. HO can be selectively detected, best at a working voltage of -0.4 V (vs. Ag/AgCl), with a 6.3 nM detection limit over the wide linear range from 50 nM to 1.0 mM. This surpasses previously reported non-enzymatic HO sensors. The sensor was successfully applied to the determination of HO in contact lens solutions and in spiked serum samples. Graphical abstract Schematic presentation of a method for electrochemical sensing of hydrogen peroxide in real samples using cobalt nanoparticle decorated tungsten carbide (WCC) modified glassy carbon electrode (GCE).
This article was published in the following journal.
Name: Mikrochimica acta
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An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 184.108.40.206.
An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC 220.127.116.11.
An enzyme that catalyzes the oxidation of cholesterol in the presence of molecular oxygen to 4-cholesten-3-one and hydrogen peroxide. The enzyme is not specific for cholesterol, but will also oxidize other 3-hydroxysteroids. EC 18.104.22.168.
An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.
A FLAVOPROTEIN, this enzyme catalyzes the oxidation of SARCOSINE to GLYCINE; FORMALDEHYDE; and HYDROGEN PEROXIDE (H2O2).
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Radiology is the branch of medicine that studies imaging of the body; X-ray (basic, angiography, barium swallows), ultrasound, MRI, CT and PET. These imaging techniques can be used to diagnose, but also to treat a range of conditions, by allowing visuali...