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The development of a simple, rapid and sensitive sensor to detect copper ion is very important for environmental detection. Here, we constructed a fluorescence biosensor based on fluorescence resonance energy transfer (FRET) between copper resistance operon coded protein C (CopC), a copper binding protein, and dansyl chloride (DNS-Cl) to selectively detect copper ion. At alkaline conditions, DNS-Cl was contently attached to CopC forming biosensor of DNS-Cl/CopC, in which fluorescence emission of CopC at 320 nm was absorbed by DNS-Cl. After binding with copper ion, the fluorescence of DNS-Cl was quenched significantly to 47%. Within the range of 0.04-11 μM (R = 0.989), the good linearity was obtained and the detection limit reached to 7 nM. More importantly, the biosensor of DNS-Cl/CopC has been successfully used to detecting of copper ion level in purified water, tap water and waste water drained from steel mill. And the results were consistent well with those obtained from the inductively coupled plasma mass spectrometry. Therefore, the established biosensor DNS-Cl/CopC is a creditable method to detect copper ion with high sensitivity and selectivity, which can be utilized as a powerful tool to monitor copper pollution in the environments.
This article was published in the following journal.
Name: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
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P-type ATPases which transport copper ions across membranes in prokaryotic and eukaryotic cells. They possess a conserved CYSTEINE-HISTIDINE-SERINE (CPx) amino acid motif within their transmembrane helices that functions in cation translocation and catalytic activation, and an N-terminal copper-binding CxxC motif that regulates enzyme activity. They play essential roles in intracellular copper homeostasis through regulating the uptake, efflux and storage of copper ions, and in cuproprotein biosynthesis.
A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.
Unstable isotopes of copper that decay or disintegrate emitting radiation. Cu atoms with atomic weights 58-62, 64, and 66-68 are radioactive copper isotopes.
Spectrophotometric techniques in which the samples are examined in the form of atoms based on their unique energy levels for ELECTRONS. They are used to analyze TRACE ELEMENTS, such as ALUMINUM; ARSENIC; BERYLLIUM; CALCIUM; COPPER; IRON; LEAD; and LITHIUM.
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