Advertisement

Topics

Toxicity assessment of wearable wound sensor constituents on keratinocytes.

08:00 EDT 27th March 2019 | BioPortfolio

Summary of "Toxicity assessment of wearable wound sensor constituents on keratinocytes."

This research reports on the cytotoxicity of materials present in a wound biosensor on human keratinocytes (HaCaT) to evaluate the biocompatibility of the sensor for continuous wound monitoring applications. Individual and collective effects of the sensor materials, gold (Au) and silver (Ag) nanoparticles (NPs), uricase enzyme (UOx), ferrocene carboxylic acid (FCA), multi-walled carbon nanotubes (MWCNTs) and poly vinyl alcohol-based polymer (PVA-SbQ) on HaCaT were studied. The toxicology profiles of these materials were derived from cell viability, mitochondrial activity retention and apoptotic behavior studies. At the concentrations present in the sensor, the cell viability studies showed minimal toxicity for Au and Ag NPs, UOx and FCA (cell viability >75%), while MWCNTs and PVA-SbQ exhibited excellent biocompatibility towards keratinocytes (cell viability >90%). Resazurin assay confirmed minimal impairment of mitochondrial activity at lower concentrations for all the materials (mitochondrial activity >0.7). The caspase-3/7 apoptotic assay showed no pronounced apoptotic behavior caused by the materials. The material mixtures studied were Au/UOx/FCA/PVA-SbQ, Ag/UOx/FCA/PVA-SbQ, and MWCNTs/UOx/FCA/PVA-SbQ. A higher toxicity profile was observed for the heterogeneous material mixtures as a result of the cumulative effect of the individual materials. However, the biosensor itself was seen to exhibit lower toxicity (~5%) compared to the material mixtures, due to the protective PVA-SbQ capping over the biosensor. This work establishes the biocompatibility of the reported wound sensor for human measurements with minimal toxic effects on human keratinocytes.

Affiliation

Journal Details

This article was published in the following journal.

Name: Toxicology in vitro : an international journal published in association with BIBRA
ISSN: 1879-3177
Pages:

Links

DeepDyve research library

PubMed Articles [11613 Associated PubMed Articles listed on BioPortfolio]

Preliminary experience of an expert panel using Triangle Wound Assessment for the evaluation of chronic wounds.

Wound assessment is an essential part of wound management and has traditionally focused on the wound bed. The Triangle of Wound Assessment (Triangle) is a new assessment tool that includes a holistic ...

Toxicity Assessment of Six Titanium Dioxide Nanoparticles in Human Epidermal Keratinocytes.

The aim of this study was to evaluate and compare the toxicity of six different types of titanium dioxide (TiO) nanoparticles (NP) on human epidermal keratinocytes (HEK).

Clinical feasibility of a wearable, conformable sensor patch to monitor motor symptoms in Parkinson's disease.

Clinical assessment of motor symptoms in Parkinson's disease (PD) is subjective and may not reflect patient real-world experience. This two-part pilot study evaluated the accuracy of the NIMBLE wearab...

SENS-U: validation of a wearable ultrasonic bladder monitor in children during urodynamic studies.

Urinary incontinence is a common problem in school-age children. Because many children remain unaware of a full-bladder sensation, the SENS-U Bladder Sensor was developed. The SENS-U is a small, weara...

A Facile Wearable Vapor/Liquid Amphibious Methanol Sensor.

Detection of methanol is a significant segment for body health and work safety in the production of chemical industry. However, there hardly exists highly selective methanol detection system with gree...

Clinical Trials [6045 Associated Clinical Trials listed on BioPortfolio]

Wearable Sensor for Biometrics During Locoregional Therapy for Head and Neck Cancer

The investigator will seek to determine the feasibility of wearable biometric sensors to acquire high resolution biometric data, including heart rate and activity level (i.e. steps) for pa...

Application of Cultured Autologous Keratinocytes for Burn Wound Healing

In this study the treatment of full thickness burn wounds with cultured autologous keratinocytes in combination with meshed split skin autograft versus meshed split skin graft alone will b...

Wearable Skin Sensors to Assess Nocturnal Scratch Behavior

Pilot study of flexible and wearable sensor to monitor nocturnal scratching behavior

Wound Dressing With Moisture Sensor

Ulcers, such as leg wounds (venous, arterial and combinations) and diabetic foot ulcers, have become a challenge for healthcare around the world. In this study we aim to assess the combina...

Validation of SHADE a Mobile Technology for Monitoring of Ultraviolet Exposure

This study evaluates the addition of a wearable real-time UV sensor in the prevention of skin cancer development in kidney-transplant patients. Half of participants will receive a wearable...

Medical and Biotech [MESH] Definitions

Electronic implements worn on the body as an implant or as an accessory. Examples include wearable diagnostic devices, wearable ACTIVITY TRACKERS, wearable INFUSION PUMPS, wearable computing devices, SENSORY AIDS, and electronic pest repellents.

The application of a vacuum across the surface of a wound through a foam dressing cut to fit the wound. This removes wound exudates, reduces build-up of inflammatory mediators, and increases the flow of nutrients to the wound thus promoting healing.

A secreted matrix metalloproteinase that may play a role in matrix degradation during WOUND HEALING. It is expressed at high levels by KERATINOCYTES, suggesting its role in keratinocyte migration.

Any process by which toxicity, metabolism, absorption, elimination, preferred route of administration, safe dosage range, etc., for a drug or group of drugs is determined through clinical assessment in humans or veterinary animals.

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Wound management
Anything that breaks the skin is a wound because when the skin is broken, there's a risk of germs getting into the body and causing an infection. Follow and track Wound Care News on BioPortfolio: Wound Car...

Enzymes
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...


Searches Linking to this Article