PM aggravates the lipid accumulation, mitochondrial damage and apoptosis in macrophage foam cells.

08:00 EDT 21st March 2019 | BioPortfolio

Summary of "PM aggravates the lipid accumulation, mitochondrial damage and apoptosis in macrophage foam cells."

Epidemiological evidence showed that the particulate matter exposure is associated with atherosclerotic plaque progression, which may be related to foam cell formation, but the mechanism is still unknown. The study was aimed to investigate the toxic effects and possible mechanism of PM on the formation of macrophage foam cells induced by oxidized low density lipoprotein (ox-LDL). Results showed that PM induced cytotoxicity by decreasing the cell viability and increasing the LDH level in macrophage foam cells. PM aggravated the lipid accumulation in ox-LDL-stimulated macrophage RAW264.7 within markedly increasing level of intracellular lipid by Oil red O staining. The level of ROS increased obivously after co-exposure to PM and ox-LDL than single exposure group. In addition, serious mitochondrial damage such as the mitochondrial swelling, cristae rupturing and disappearance were observed in macrophage foam cells. The loss of the mitochondrial membrane potential (MMP) further exacerbated the mitochondrial damage in PM-induced macrophage foam cells. The apoptotic rate increased more severely via up-regulated protein level of Bax, Cyt C, Caspase-9, Caspase-3, and down-regulated that of Bcl-2, indicating that PM activated the mitochondrial-mediated apoptosis pathway. In summary, our results demonstrated that PM aggravated the lipid accumulation, mitochondrial damage and apoptosis in macrophage foam cells, suggesting that PM was a risk factor of atherosclerosis progression.


Journal Details

This article was published in the following journal.

Name: Environmental pollution (Barking, Essex : 1987)
ISSN: 1873-6424
Pages: 482-490


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