Advertisement

Topics

Accelerating structural life science by paramagnetic lanthanide probe methods.

08:00 EDT 27th March 2019 | BioPortfolio

Summary of "Accelerating structural life science by paramagnetic lanthanide probe methods."

We describe the recent progress of structural analysis methods exploiting paramagnetic lanthanide ions. In NMR spectroscopy, the paramagnetic effects induced by the trivalent lanthanide ions provide long-range (~40 Å) distance and angular information that can be exploited in protein structure determination, ligand screening, structure-based resonance assignment, and in-cell observation. The paramagnetic lanthanide ions can also be utilized in EPR spectroscopy, providing nanometer-scale distance measurement. These applications of the paramagnetic lanthanide probe are becoming more widespread by the use of a variety of lanthanide binding tags. Here, we introduce the basics of paramagnetic effects, several examples of lanthanide tags, and recent applications of paramagnetic lanthanide ions in NMR and EPR spectroscopy. Collectively, we show how the paramagnetic lanthanide probe accelerates research in protein science and drug design, and consequently life science.

Affiliation

Journal Details

This article was published in the following journal.

Name: Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Pages:

Links

DeepDyve research library

PubMed Articles [24355 Associated PubMed Articles listed on BioPortfolio]

Ln(III) chelates-functionalized carbon quantum dots: Synthesis, optical studies and multimodal bioimaging applications.

The carbon quantum dots termined with amine groups (CQDs-NH) were synthesized in one-pot hydrothermal method with citric acid as carbon source and branched polyethylenimine (BPEI) as passive agent, wh...

Magnetic Circular Dichroism Spectroscopy of Metalloproteins.

Metals and metal clusters in proteins typically serve as important structural/functional motifs. Because of this reason, there is a wide range of techniques that specifically probe the structure and e...

Construction, characteristics, and constraints of accelerating beams based on caustic design.

Caustic methods have been proposed for wavefront design to enable light beams propagating along curved trajectories, namely accelerating beams. Here we elaborate the complete construction, remarkable ...

Eu as a luminescence probe in DNA studies: Structural and conformational implications.

Lanthanide ions are widely used as luminescent probes for structural studies of various biomolecules, including DNA. Latest developments of circularly polarized luminescence (CPL) methodology further ...

EPR-Derived Structure of a Paramagnetic Intermediate Generated by Biotin Synthase BioB.

Biotin (vitamin B) is an enzyme cofactor required by organisms from all branches of life but synthesized only in microbes and plants. In the final step of biotin biosynthesis, a radical S-adenosyl-L-m...

Clinical Trials [5517 Associated Clinical Trials listed on BioPortfolio]

Pilot Study In Vivo Confocal Microscopy Probe for Cervical Precancer

The goal of this proposal is to determine whether a hand-held confocal microscopy probe may perform better than standard methods in detecting cancer or pre-cancerous abnormalities of the c...

The Impact of a Modified Probe Tip Design on Patient Perception of Discomfort on Probing and Clinical Parameters Using the Florida Probe® System

Patient perception of pain on periodontal probing using a controlled-force, electronic probe was not significantly affected by the use of a modified (ball-end) probe tip design except for ...

Improving STEM Outcomes for Young Children With Language Learning Disabilities

The sophisticated language of science can be a barrier to Science, Technology, Engineering, and Math (STEM) learning, especially for children who have specific language impairment (SLI). T...

Measurement of the Partial Pressure of Oxygen in Cutaneous Tumors Using Electron Paramagnetic Resonance (EPR) Oximetry

This protocol will take measurements of a variety of tumors involving the skin in order to assess changes in tumor oxygen from hyperoxygenation therapy and standard cancer-directed treatme...

Six Months Evaluation of Next Science Acne Creams on Facial Acne in Adult Females.

To assess facial acne vulgaris clinical and psychological outcomes by measuring differences in lesions counts, investigator global assessment, and subjects quality of life scores over 6 mo...

Medical and Biotech [MESH] Definitions

Devices for accelerating protons or electrons in closed orbits where the accelerating voltage and magnetic field strength varies (the accelerating voltage is held constant for electrons) in order to keep the orbit radius constant.

The traditional Hindu system of medicine which is based on customs, beliefs, and practices of the Hindu culture. Ayurveda means "the science of Life": veda - science, ayur - life.

The field of knowledge, theory, and technology dealing with the collection of facts and figures, and the processes and methods involved in their manipulation, storage, dissemination, publication, and retrieval. It includes the fields of COMMUNICATION; PUBLISHING; LIBRARY SCIENCE; and informatics.

The science dealing with the earth and its life, especially the description of land, sea, and air and the distribution of plant and animal life, including humanity and human industries with reference to the mutual relations of these elements. (From Webster, 3d ed)

A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Bioscience
Bioscience - any of the sciences that deal with living organisms.  The study of the nature, behavior, and uses of living organisms as applied to biology. Any of the branches of natural science dealing with living things, such as their structure, b...

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...


Searches Linking to this Article