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Layer-by-layer assembly of enzyme-loaded halloysite nanotubes for the fabrication of highly active coatings.

08:00 EDT 21st March 2019 | BioPortfolio

Summary of "Layer-by-layer assembly of enzyme-loaded halloysite nanotubes for the fabrication of highly active coatings."

The development of sturdy enzyme-containing hydrophilic coatings is important for applications such as water purification or biological sensing. Here, we investigate the encapsulation of a model enzyme (beta-lactamase, BlaP) into aluminosilicate halloysite nanotubes (HNTs), and their subsequent use for the fabrication of enzymatic coatings by layer-by-layer (LbL) assembly. Highly stable suspensions of enzymatically-active halloysite nanotubes were obtained by alkaline treatment of HNTs, followed by enzyme adsorption into the lumen of the nanotubes and of poly(ethylene imine) (PEI) onto their outer surface. Bioactive thin films based on the LbL-assembly of these modified nanotubes with negatively-charged alginate provided coatings with a significantly higher enzymatic activity compared to films in which the enzyme is not incorporated in the nanotubes. The obtained results show that the encapsulation of an enzyme in halloysite nanotubes is a viable route towards stable bioactive coatings, which could be easily adapted to entrap other types of biomacromolecules with the aim of preparing thin films for air or effluent decontamination.

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This article was published in the following journal.

Name: Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Pages: 508-514

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