Tuning surface functionalization and collagen gel thickness to regulate cancer cell migration.

08:00 EDT 16th March 2019 | BioPortfolio

Summary of "Tuning surface functionalization and collagen gel thickness to regulate cancer cell migration."

Cancer cells have a tremendous ability to sense and respond to extracellular matrix (ECM) stiffness, modulating invasion. The magnitude of the sensed stiffness can either promote or inhibit the migration of cancer cells out of the primary tumor into surrounding tissue. Work has been done on examining the role of stiffness in tuning cancer cell migration by controlling elastic modulus in the bulk. However, a powerful and complementary approach for controlling stiffness is to leverage interactions between stiff-soft (e.g. glass-hydrogel) interfaces. Unfortunately, most work in this area probes cells in 2D environments. Of the reports that probe 3D environments, none have assessed the role of mechanical linkage to the interface as a potential handle in controlling local stiffness and cell behavior. In this paper, we examine the migration of cancer cells embedded in a collagen fiber network between two flat plates. We examine the role of both surface attachment of the collagen network to the stiff interface as well as thickness (50-540 μm) of the collagen gel in driving collagen organization, cell morphology and cell migration. We find that surface attachment and thickness do not operate overlapping mechanisms, because they elicit different cell responses. While thickness and surface chemistry appear to control morphology, only thickness regulates collagen organization and cell migration speed. This suggests that surface attachment and thickness of the collagen gel control cell behavior through both collagen structure and local stiffness in confined fiber-forming networks.


Journal Details

This article was published in the following journal.

Name: Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Pages: 37-47


DeepDyve research library

PubMed Articles [36166 Associated PubMed Articles listed on BioPortfolio]

Membrane associated collagen XIII promotes cancer metastasis and enhances anoikis resistance.

Increased collagen expression and deposition are associated with cancer progression and poor prognosis in breast cancer patients. However, function and regulation of membrane-associated collagen in br...

Osteoblast adhesion and response mediated by terminal -SH group charge surface of SiOxCy nanowires.

Robust cell adhesion is known to be necessary to promote cell colonization of biomaterials and differentiation of progenitors. In this paper, we propose the functionalization of Silicon Oxycarbide (Si...

A structural prospective for collagen receptors such as DDR and their binding of the collagen fibril.

The structure of the collagen fibril surface directly effects and possibly assists the management of, collagen receptor interactions. An important class of collagen receptors, the receptor tyrosine ki...

Effect of functionalized gold nanoparticle on collagen stabilization for tissue engineering application.

Functionalization of nanoparticle with specific groups is one of the most straightforward strategies to induce structural stability and specific cell responses from collagen based biomaterials. The ef...

A proteomics study to explore the role of adsorbed serum proteins for PC12 cell adhesion and growth on chitosan and collagen/chitosan surfaces.

The aim of this article is to apply proteomics in the comparison of the molecular mechanisms of PC12 cell adhesion and growth mediated by the adsorbed serum proteins on the surfaces of chitosan and co...

Clinical Trials [20057 Associated Clinical Trials listed on BioPortfolio]

Study of Collagen IV and XIX in Bronchoalveolar Lavage, Pulmonary Aspiration and Bronchial Biopsies

Collagens are proteins present in all tissues. Besides their structural role, recent data showed that they were able to regulate many cellular functions. Many interactions occur between ex...

Topical Collagen Powder for Healing of Acute Full-thickness Wounds

We have designed a pilot study to investigate the effect and potential utility of topical NuvagenTM (collagen powder) on the rate and quality of wound healing in healthy volunteers using t...

Contribution of PRF in CDH in Children With Prothetic Patch Closure

Improved management of giant congenital diaphragmatic hernias (CDH) in neonates : decreased risk of morbidity and mortality due to prosthesis release. CDH is a rare disease with a still ve...

Corneal Collagen Crosslinking for Progressive Keratoconus and Ectasia Using Riboflavin/Dextran and Hypotonic Riboflavin

Corneal collagen crosslinking (CXL) has been proposed as an effective method of reducing progression of both keratoconus and corneal ectasia after surgery, as well as possibly decreasing t...

Clinical Study of Umbilical Cord Mesenchymal Stem Cells Combined With Collagen Scaffold in the Treatment of Thin Endometrium

This is a non-blind self-control trial. We mainly compare the changes of endometrial thickness,changes in menstrual flow, and improvement in pregnancy before and after stem cell treatmen...

Medical and Biotech [MESH] Definitions

Collagen receptors are cell surface receptors that modulate signal transduction between cells and the EXTRACELLULAR MATRIX. They are found in many cell types and are involved in the maintenance and regulation of cell shape and behavior, including PLATELET ACTIVATION and aggregation, through many different signaling pathways and differences in their affinities for collagen isoforms. Collagen receptors include discoidin domain receptors, INTEGRINS, and glycoprotein VI.

Cell surface receptor for LAMININ, epiligrin, FIBRONECTINS, entactin, and COLLAGEN. Integrin alpha3beta1 is the major integrin present in EPITHELIAL CELLS, where it plays a role in the assembly of BASEMENT MEMBRANE as well as in cell migration, and may regulate the functions of other integrins. Two alternatively spliced isoforms of the alpha subunit (INTEGRIN ALPHA3), are differentially expressed in different cell types.

A fibril-associated collagen usually found crosslinked to the surface of COLLAGEN TYPE II fibrils. It is a heterotrimer containing alpha1(IX), alpha2(IX) and alpha3(IX) subunits.

A large superfamily of cell surface membrane proteins characterized by their four transmembrane domains. They play a role in a variety of processes such as cellular adhesion and motility. They may be involved in the organization of cell surface MEMBRANE MICRODOMAINS that regulate the activation of LEUKOCYTES.

Functionalization of exogenous substances to prepare them for conjugation in PHASE II DETOXIFICATION. Phase I enzymes include CYTOCHROME P450 enzymes and some OXIDOREDUCTASES. Excess induction of phase I over phase II detoxification leads to higher levels of FREE RADICALS that can induce CANCER and other cell damage. Induction or antagonism of phase I detoxication is the basis of a number of DRUG INTERACTIONS.

Quick Search


DeepDyve research library

Relevant Topics

Cancer Disease
Cancer is not just one disease but many diseases. There are more than 100 different types of cancer. Most cancers are named for the organ or type of cell in which they start - for example, cancer that begins in the colon is called colon cancer; cancer th...

  Bladder Cancer Brain Cancer Breast Cancer Cancer Cervical Cancer Colorectal Head & Neck Cancers Hodgkin Lymphoma Leukemia Lung Cancer Melanoma Myeloma Ovarian Cancer Pancreatic Cancer ...

Searches Linking to this Article