Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol.

08:00 EDT 16th March 2019 | BioPortfolio

Summary of "Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol."

This study reports engineering of a hypertransformable variant of C. pasteurianum for bioconversion of glycerol into hydrogen (H). A functional glycerol-triggered hydrogen pathway was engineered based on two approaches: (1) increasing product yield by overexpression of immediate enzyme catalyzing H production, (2) increasing substrate uptake by overexpression of enzymes involved in glycerol utilization. The first strategy aimed at overexpression of hydA gene encoding hydrogenase, and the second one, through combination of overexpression of dhaD1 and dhaK genes encoding glycerol dehydrogenase and dihydroxyacetone kinase. These genetic manipulations resulted in two recombinant strains (hydA/dhaD1K) capable of producing 97% H (v/v), with yields of 1.1 mol H/mol glycerol in hydA overexpressed strain, and 0.93 mol H/mol glycerol in dhaD1K overexpressed strain, which was 1.5 fold higher than wild type. Among two strains, dhaD1K consumed more glycerol than hydA which proves that overexpression of glycerol enzymes has enhanced glycerol intake rate.


Journal Details

This article was published in the following journal.

Name: Bioresource technology
ISSN: 1873-2976
Pages: 168-177


DeepDyve research library

PubMed Articles [6767 Associated PubMed Articles listed on BioPortfolio]

Enhanced electron transfer of different mediators for strictly opposite shifting of metabolism in Clostridium pasteurianum grown on glycerol in a new electrochemical bioreactor.

Microbial electrosynthesis or electro-fermentation in bioelectrochemical systems (BES) have recently received much attention. Here we demonstrate with the glycerol metabolism by Clostridium pasteurian...

Stimulation of ferrihydrite nanorods on fermentative hydrogen production by Clostridium pasteurianum.

Conversion of organic matter to biohydrogen possesses promising application potential. In this study, low-cost ferrihydrite nanorods were used to enhance hydrogen production by Clostridium pasteurianu...

Production of 1,3-propanediol from pure and crude glycerol using a UASB reactor with attached biomass in silicone support.

The 1,3-propanediol (1,3-PDO) yield and productivity from glycerol were studied over a 155-day period. A UASB reactor that also contained silicone support for biomass attachment was used to evaluate t...

Clostridium butyricum population balance model: Predicting dynamic metabolic flux distributions using an objective function related to extracellular glycerol content.

Extensive experimentation has been conducted to increment 1,3-propanediol (PDO) production using Clostridium butyricum cultures in glycerol, but computational predictions are limited. Previously, we r...

Dihydroxyacetone Production from Glycerol using Gluconobacter oxydans: Study of Medium Composition and Operational Conditions in Shaken Flasks.

The production of dihydroxyacetone from glycerol employing aerobic cultures of Gluconobacter oxydans is studied. Dihydroxyacetone is one of the most important value-added products obtained from glycer...

Clinical Trials [1650 Associated Clinical Trials listed on BioPortfolio]

Clostridium Difficile Vaccine Efficacy Trial (Clover)

The Clover trial is evaluating an investigational vaccine that may help to prevent Clostridium difficile infection. Participants in the study are adults 50 years of age and older, who are ...

Efficacy Study of Oxygenated Glycerol Triesters to Treat Xerostomia

The objective of the trial was to evaluate the efficacy and clinical safety and acceptability of Oxygenated Glycerol Triesters in the relief of symptoms of xerostomia versus a reference co...

Clostridium Difficile Vaccine 2-Dose Versus 3-Dose Study

This study will investigate a Clostridium difficile vaccine in healthy adults 50 years of age and older. In half the healthy adults, all 3 doses given are the Clostridium difficile vaccine...

Comparative Study of Three Different Testing Mechanisms for Clostridium Difficile

The purpose of this study is to establish which of the following tests perform best in diagnosing clostridium difficile. PCR, Enzyme Immunoassays (EIA) and C. difficile cytotoxin assay (C...

Acute Effects of Propylene Glycol/Glycerol Intake on Blood Parameters

The aim of this study is to evaluate the acute effect of propylene glycol and glycerol intake on cardiorespiratory blood parameters.

Medical and Biotech [MESH] Definitions

A non-heme iron-sulfur protein isolated from Clostridium pasteurianum and other bacteria. It is a component of NITROGENASE along with molybdoferredoxin and is active in nitrogen fixation.

A non-heme iron-sulfur protein isolated from Clostridium pasteurianum and other bacteria. It is a component of NITROGENASE, which is active in nitrogen fixation, and consists of two subunits with molecular weights of 59.5 kDa and 50.7 kDa, respectively.

An NAD-dependent enzyme that catalyzes the oxidation of sn-glycerol 3-phosphate to glycerone phosphate.

An enzyme that catalyzes the formation of glycerol 3-phosphate from ATP and glycerol. Dihydroxyacetone and L-glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. EC

A species of gram-positive bacteria in the family Clostridiaceae. Its GLUTAMATE DEHYDROGENASE is commonly used in research.

Quick Search


DeepDyve research library

Relevant Topics

Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Recombinant DNA
Recombinant DNA is the formation of a novel DNA sequence by the formation of two DNA strands. These are taken from two different organisms. These recombinant DNA molecules can be made with recombinant DNA technology. The procedure is to cut the DNA of ...

Searches Linking to this Article