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A detailed study of the effect of aprotic polar solvents such as dimethylsulfoxide on methylene blue (MB) was carried out through a combination of UV-vis absorption and steady-state fluorescence spectroscopy techniques at 293.15 K. In aqueous solutions MB tends to exhibit strong tendency to aggregate. The dimerization behavior of MB in water was analyzed in terms of monomer-dimer equilibrium. The addition of dimethylsulfoxide prevents dimerization of dye molecules. From absorption spectra the dimerization constants and changes of standard Gibbs energy were calculated. From the steady-state fluorescence spectra the quantum yields and Stokes shifts were determined. To explain the effect of dimethylsulfoxide properly the other polar aprotic solvents such as N, N-dimethylformamide, acetonitrile and acetone were used. It is suggested that water structural effect is the major factor in aggregation phenomenon. Moreover the quantum yield increases drastically in dimethylsulfoxide compared with water assuming that MB may be characterized as viscosity probe.
This article was published in the following journal.
Name: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
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A compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene blue is used as a bacteriologic stain and as an indicator. It inhibits GUANYLATE CYCLASE, and has been used to treat cyanide poisoning and to lower levels of METHEMOGLOBIN.
Sterile solutions, essentially free from foreign particles and suitably compounded and dispensed, for instillation into the eye. It does not include solutions for cleaning eyeglasses or CONTACT LENS SOLUTIONS. (From Dorland, 27th ed)
Local antibacterial that probably acts by releasing formaldehyde in aqueous solutions. It is used for irrigation of infected body cavities - bladder, peritoneum, etc. and as a spray for burns.
Usually a benign tumor, that commonly presents as a solitary blue nodule with spindled MELANOCYTES covered by smooth SKIN. Several variants have been identified, one variant being malignant. The blue color is caused by large, densely packed melanocytes deep in the DERMIS of the nevus. In CHILDREN, they usually occur on the BUTTOCKS and LUMBOSACRAL REGION and are referred to as cellular blue nevi. Malignant blue nevi are more commonly found on the SCALP.
A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.