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As a new class of two-dimensional (2D) materials, molybdenum disulfide (MoS) has huge potential in biomedical area; while its applications in multiplex bioassays are still a challenge. Here, we present novel MoS-integrated silica colloidal crystal barcode (SCCB) for multiplex microRNA (miRNA) screening. MoS was adsorbed on SCCBs by electrostatic interaction, and quantum dots (QDs) decorated hairpin probes were coupled on MoS by covalent linkage. As the MoS could quench the QDs of the hairpin probes, they together formed a molecular beacon (MB) structure before the detection. When used in assays, target miRNA could form a double strand with the probe and made QDs keep away from MoS sheets to recovery their fluorescence. Because the released QDs were positively correlated with the concentration of the hybridized nucleic acid, the target miRNAs could be quantified by measuring the fluorescence signal of the QDs on the SCCBs. In addition, by utilizing different MoS-integrated structural color encoded SCCBs, multiplexed miRNA quantification could also be realized. Based on this strategy, we have demonstrated that several pancreatic cancer-related miRNAs could be selectivity and sensitivity detected with a detection limit of 4.2 ± 0.3 nM. These features make the MoS-integrated SCCB ideal for many potential applications.
This article was published in the following journal.
Name: Biosensors & bioelectronics
Recently, molybdenum disulfide (MoS) as a new type of catalyst, has been used for hydrogen evolution. In order to overcome the shortcomings of MoS, such as poor conductivity and lack of catalytic acti...
In this study, we report a potentially scalable method for producing multilayer molybdenum disulfide (MoS) nanosheets. The addition of a small amount of ammonia solution can improve the exfoliation of...
A metal-inorganic composite, comprises of silver-molybdenum disulfide nanosheets (Ag@MoS) was synthesized at low temperature. The Ag@MoS composite was drop-casted onto a glassy carbon electrode (GCE) ...
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To evaluate the safety and efficacy of ALXN1101 in neonate patients with MoCD Type A
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The effects of Minimal Flow Anesthesia (0.4 l / min) and High Flow Anaesthesia (2 l / min) on tissue oxygen saturation (St02) and thiol / disulfide balance in hypotensive anesthesia operat...
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (TUMOR MARKERS, BIOLOGICAL) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
An integrated professional approach to screening, evaluation, control, and reduction of abnormal WEIGHT GAIN.
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Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
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A microRNA (abbreviated miRNA) is a small non-coding RNA molecule (containing about 22 nucleotides) found in plants, animals, and some viruses. Key findings: miRNA is involved in the normal functioning of eukaryotic cells, so has dysregulation...
Immunoassay - ELISA
Immunoassays are quick and accurate tests to detect specific molecules. Immunoassays rely on an antibody to bind to the specific structure of a molecule. Antibodies are proteins generated by animals in response to the invasion of a foreign molecule (anti...